A genetically encoded Mango II-based system with increased brightness and selectivity for imaging RNAs in live cells

IF 2.7 3区化学 Q1 CHEMISTRY, ORGANIC

Organic & Biomolecular Chemistry Pub Date : 2025-07-10 DOI:10.1039/d5ob01004g

Julia I. Svetlova , Oksana S. Bychenko , Georgy K. Slushko , Yulia V. Skvortsova , Evgeny S. Belyaev , Polina N. Kamzeeva , Yuriy M. Khodarovich , Alexey A. Pakhomov , Tatyana L. Azhikina , Anna M. Varizhuk , Andrey V. Aralov

引用次数: 0

Abstract

Few genetically encoded systems based on aptamer–dye pairs meet all the requirements of bioimaging in terms of brightness, contrast, etc., thus necessitating their optimization. Here, we report a new biotin-free fluorogenic ligand that demonstrates increased selectivity for Mango II over other DNA/RNA structures in tube and brightness in complex with genetically encoded aptamer-labeled RNA in live cells compared to the known dye.

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一种基于芒果ii的遗传编码系统，具有更高的亮度和选择性，用于成像活细胞中的rna。

很少有基于适体-染料对的遗传编码系统在亮度、对比度等方面满足生物成像的所有要求，因此需要对其进行优化。在这里，我们报道了一种新的无生物素荧光配体，与已知染料相比，它在试管中比其他DNA/RNA结构和活细胞中遗传编码的适体标记RNA复合物中具有更高的选择性和亮度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.