Development of a User-Friendly Dynamic Culture System for Decellularized Scaffold-Based Tissue Engineering.

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2025-08-29 DOI:10.1021/acsbiomaterials.5c01129

Shujuan Fan, Yerong Qian, Dan Li, Lu Lu, Jie Lian, Xin Liu, Junxi Xiang, Aihua Shi, Shasha Wei, Yi Lyu, Lifei Yang, Peng Liu

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引用次数: 0

Abstract

Background: The engineering of large-scale tissues is frequently hampered by complex, inefficient perfusion bioreactors and the inherent diffusion limits of static culture. To overcome these operational and biological barriers, we developed and validated a novel, user-friendly dynamic culture platform.

Methods: Our miniaturized platform seamlessly integrates a peristaltic pump, an oxygenator, and perfusion circuits into a single, easy-to-assemble unit with smart control. We demonstrated its efficacy by culturing AML12 hepatocytes on decellularized rat liver scaffolds for 7 days and comparing its performance against static culture controls.

Results: Dynamic culture dramatically enhanced cell viability and functional maturity. Compared to static controls, constructs cultured in our platform exhibited a > 5-fold increase in proliferating (Ki67+) cells and a 32-fold decrease in apoptotic (TUNEL+) cells. Hepatic functions were also significantly enhanced, with increased urea production and markedly higher expression of albumin (∼1.5-fold) and the key metabolic enzymes UGT1 (∼1.4-fold) and CYP2D6 (∼2.7-fold).

Conclusion: Our integrated dynamic culture platform provides a simple, reliable, and effective solution for constructing large, viable 3D tissue constructs. By overcoming critical usability and mass transport challenges, this platform represents a powerful and scalable tool for advancing tissue engineering applications, from regenerative medicine to disease modeling.

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基于支架的组织工程脱细胞动态培养系统的开发。

背景：大规模组织的工程常常受到复杂、低效的灌注生物反应器和静态培养固有的扩散限制的阻碍。为了克服这些操作和生物障碍，我们开发并验证了一个新颖的、用户友好的动态文化平台。方法：我们的小型平台将蠕动泵、氧合器和灌注电路无缝集成到一个易于组装的智能控制单元中。我们通过在去细胞化的大鼠肝支架上培养AML12肝细胞7天，并将其性能与静态培养对照进行比较，证明了其有效性。结果：动态培养显著提高细胞活力和功能成熟度。与静态对照相比，在我们的平台上培养的构建物显示增殖（Ki67+）细胞增加了bb50倍，凋亡（TUNEL+）细胞减少了32倍。肝功能也显著增强，尿素产量增加，白蛋白（~ 1.5倍）和关键代谢酶UGT1（~ 1.4倍）和CYP2D6（~ 2.7倍）的表达显著增加。结论：我们的集成动态培养平台为构建大型、有活力的三维组织构建体提供了简单、可靠、有效的解决方案。通过克服关键的可用性和大规模运输挑战，该平台代表了一个强大且可扩展的工具，用于推进组织工程应用，从再生医学到疾病建模。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture