Cu-MOF@TMB Integrated Test Strips for Naked Eye Colorimetric Detection of a Critical Biomarker for Metabolic and Renal Disorders

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-08-25 DOI:10.1021/acsabm.5c01198

Preeti Sharma, Musheer Ahmad, Nazrul Haq, Devendra Dewangan, Raghavender Medishetty, Anil Kumar Astakala, Nohyun Lee and Kafeel Ahmad Siddiqui*,

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引用次数: 0

Abstract

Uric acid (UA) serves as a vital biomarker for diagnosing metabolic and renal disorders, prompting the need for rapid and selective detection methods. Herein, a new copper-based metal–organic framework (Cu-MOF), formulated as [Cu₂(TCi)₂] [Cu₅(TCi)₂(H₂O)₈(μ₃–OH)₂], was synthesized using Cu(II) ions and tris(2-carboxyethyl) isocyanurate (H₃TCi) as a flexible linker. Comprehensive characterization through PXRD, FTIR, SEM, EDAX, TGA, XPS, BET and elemental mapping confirmed the framework’s structural integrity. To enhance sensing performance, the MOF was integrated with 3,3′,5,5′-tetramethylbenzidine (TMB), forming Cu-MOF@TMB composites. Among them, Cu-MOF@TMB-1 exhibited the highest sensitivity, achieving a limit of detection (LOD) of 0.941 ppm in aqueous solution, which further improved to 0.235 ppm in model urine and 0.149 ppm in real urine samples. A portable, enzyme-free, test strip-based colorimetric assay enabled rapid, visible detection of UA through a distinct blue color change. This work demonstrates a cost-effective and selective MOF-based sensing platform with strong potential for clinical diagnostics and point-of-care applications.

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Cu-MOF@TMB用于代谢和肾脏疾病的关键生物标志物的裸眼比色检测的集成测试条。

尿酸（UA）作为诊断代谢和肾脏疾病的重要生物标志物，促使对快速和选择性检测方法的需求。本文以Cu（II）离子和三（2-羧基乙基）异氰尿酸酯（H3TCi）为柔性连接剂，合成了一种新型铜基金属有机骨架（Cu- mof），分子式为[Cu2(TCi)2] [Cu5(TCi)2(H2O)8(μ3-OH)2]。通过PXRD， FTIR， SEM， EDAX， TGA， XPS， BET和元素映射等综合表征证实了该框架的结构完整性。为了提高传感性能，将MOF与3,3',5,5'-四甲基联苯胺（TMB）集成，形成Cu-MOF@TMB复合材料。其中Cu-MOF@TMB-1灵敏度最高，水溶液的检出限（LOD）为0.941 ppm，模型尿液的检出限为0.235 ppm，真实尿液样品的检出限为0.149 ppm。一种便携式、无酶、基于测试条的比色法，通过明显的蓝色变化，可以快速、可见地检测UA。这项工作证明了一种具有成本效益和选择性的基于mof的传感平台，具有临床诊断和护理点应用的强大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.