Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-09-03 DOI:10.1126/sciadv.adw7764

Yang Su, Maiya Callender, Dylan Woolsey, Joanna Zhu, Colleen Sedney, Mimi Thu Vu, Jillian Masters, Amanda Caulfield, Kalyan Dewan, Emily Gaskill, Reilly Stevens, Austin Joshua, Christopher M. Evans, John Glushka, Evgeny Vinogradov, Andrew Preston, Thomas Krunkosky, Maor Bar-Peled, Eric T. Harvill

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Abstract

Respiratory tract infections pose considerable global health challenges. Upper airway colonization is pivotal to these infections, including those caused by Bordetella species. We identified an oligosaccharide, bordetellae colonization oligosaccharide (b-Cool), crucial for early nasal colonization of Bordetella bronchiseptica. We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including Bordetella pertussis, which causes whooping cough in humans. A B. bronchiseptica mutant lacking b-Cool (Δb-Cool) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of Δb-Cool was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.

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博德tellae定植寡糖（b-Cool），一种对鼻腔定植至关重要的聚糖

呼吸道感染对全球健康构成相当大的挑战。上呼吸道定植对这些感染至关重要，包括由博德特拉菌引起的感染。我们鉴定了一种寡糖，博德tellae定植寡糖（b-Cool），对支气管博德tella早期鼻腔定植至关重要。我们通过LC-MS和NMR表征了b-Cool的结构，并发现它在多种博德tellae中普遍存在，包括导致人类百日咳的百日咳博德tellae。缺乏b-Cool的支气管结核分枝杆菌突变体（Δb-Cool）在小鼠鼻咽和鼻上皮中的定植明显延迟和减少，导致传播减少。在粘蛋白缺乏的小鼠中，Δb-Cool的定殖缺陷被修复，这表明b-Cool可能在气道粘蛋白存在的情况下促进定植。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.