Hsa_circ_0005325 Regulates the Proliferation, Apoptosis, Colony Formation, Migration, and Angiogenesis-Promoting Behavior of Oral Squamous Cell Carcinoma Cells Through the miR-433-3p/HMGA2 Axis

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2025-09-03 DOI:10.1002/cre2.70208

Zhihan Lin, Yating Fu, Lei Mao, Hongjuan Yan, Wen Liu, Xiaoxue Tang

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Abstract

Objective

To explore the mechanism by which hsa_circ_0005325 affects the proliferation, apoptosis, colony formation, migration, and angiogenesis-promoting behavior of oral squamous cell carcinoma cells through the miR-433-3p/HMGA2 axis.

Material and Methods

qRT‒PCR was used to measure the expression of hsa_circ_0005325 in SCC25 and CAL-27 cells and normal human oral epithelial cells (HOK). SCC25 and CAL-27 cells were cultured, and Cell Counting Kit-8 (CCK-8), cell apoptosis, plate colony formation, Transwell migration and a tube formation assays were used to detect changes in cell proliferation, apoptosis, colony formation, migration and angiogenesis, respectively.

Results

The expression of hsa_circ_0005325 was significantly increased in SCC25 and CAL-27 cells. Compared with those in the sh-NC group, the percentages of apoptotic SCC25 and CAL-27 cells in the sh-circ_0005325 group were significantly greater, and their proliferation, colony formation, migration and angiogenesis capacities were significantly lower (p < 0.05). Moreover, the protein expression level of HMGA2 was significantly decreased, and the expression level of miR-433-3p was significantly increased in the sh-circ_0005325 group versus the control group (p < 0.05).

Conclusion

Hsa_circ_0005325 is highly expressed in SCC25 and CAL-27 cells. Downregulation of hsa_circ_0005325 can inhibit the proliferation and invasion of SCC25 and CAL-27 cells and promote their apoptosis.

Abstract Image

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Hsa_circ_0005325通过miR-433-3p/HMGA2轴调控口腔鳞状细胞癌细胞的增殖、凋亡、集落形成、迁移和促血管生成行为

目的探讨hsa_circ_0005325通过miR-433-3p/HMGA2轴影响口腔鳞状细胞癌细胞增殖、凋亡、集落形成、迁移和促血管生成行为的机制。材料与方法采用qRT-PCR方法检测hsa_circ_0005325在SCC25、CAL-27细胞和正常人口腔上皮细胞（HOK）中的表达。培养SCC25和CAL-27细胞，采用细胞计数试剂盒-8 （CCK-8）、细胞凋亡、平板集落形成、Transwell迁移和a管形成实验分别检测细胞增殖、细胞凋亡、集落形成、迁移和血管生成的变化。结果hsa_circ_0005325在SCC25和CAL-27细胞中的表达明显升高。与sh-NC组相比，sh-circ_0005325组SCC25和CAL-27细胞凋亡百分比显著增加，增殖、集落形成、迁移和血管生成能力显著降低（p < 0.05）。sh-circ_0005325组HMGA2蛋白表达水平显著降低，miR-433-3p表达水平显著升高（p < 0.05）。结论Hsa_circ_0005325在SCC25和CAL-27细胞中高表达。下调hsa_circ_0005325可抑制SCC25和CAL-27细胞的增殖和侵袭，促进其凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.