Enhancing phenolic and flavonoid recovery from Vietnamese balm using green solvent-based ultrasonic-enzymatic-assisted extraction

IF 9.7 1区 化学 Q1 ACOUSTICS
Tan Phat Vo , Thi Hoang Trang Nguyen , Ha Bao Tran Nguyen , Hoang Nhan Nguyen , Nguyen Van Nhi Le , Minh Hoa Ha , Gia Bao Pham , Dinh Quan Nguyen
{"title":"Enhancing phenolic and flavonoid recovery from Vietnamese balm using green solvent-based ultrasonic-enzymatic-assisted extraction","authors":"Tan Phat Vo ,&nbsp;Thi Hoang Trang Nguyen ,&nbsp;Ha Bao Tran Nguyen ,&nbsp;Hoang Nhan Nguyen ,&nbsp;Nguyen Van Nhi Le ,&nbsp;Minh Hoa Ha ,&nbsp;Gia Bao Pham ,&nbsp;Dinh Quan Nguyen","doi":"10.1016/j.ultsonch.2025.107546","DOIUrl":null,"url":null,"abstract":"<div><div>The research explored the extraction of phenolic and flavonoids from <em>Elsholtzia ciliata</em> (Vietnamese balm) using a natural deep eutectic solvent (NADES)-based ultrasonic-enzymatic-assisted extraction (UAE-EAE) technique. A range of NADESs was evaluated to investigate the most suitable solvent system. The NADES synthesized from lactic acid and choline chloride provided the highest recovery of phenolics and flavonoids. Single-factor experiments were conducted to assess how individual process variables influence the recovery of phenolics and flavonoids. Key factors affecting extraction performance were assessed using the Plackett-Burman approach. Optimal conditions were established through the Box-Behnken Design model. The optimal extraction parameters for TPhC were determined as a solid-to-liquid ratio (SLR) of 1:59 g/mL, water content (WC) of 12 %, ultrasonic temperature of 43 °C, ultrasonic time of 6.88 min, and incubation time of 6.33 min. For TFlC, the optimal conditions were established at SLR of 1:67 g/mL, WC of 14 %, and enzyme activity of 1.28 U/g. Under these optimized conditions, UAE-EAE achieved the maximum recovery of total phenolic content (36.41 ± 2.50 mg GAE/g) and total flavonoid content (18.74 ± 1.28 mg QE/g). A second-order kinetic model was adopted to analyze the extraction mechanism. Structural and surface changes in the plant matrix before and after extraction were further investigated using scanning electron microscopy (SEM) and X-ray diffraction (XRD). This method demonstrates high efficiency and eco-friendliness in extracting plant-derived bioactive compounds using NADES-based UAE-EAE.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"121 ","pages":"Article 107546"},"PeriodicalIF":9.7000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasonics Sonochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1350417725003256","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ACOUSTICS","Score":null,"Total":0}
引用次数: 0

Abstract

The research explored the extraction of phenolic and flavonoids from Elsholtzia ciliata (Vietnamese balm) using a natural deep eutectic solvent (NADES)-based ultrasonic-enzymatic-assisted extraction (UAE-EAE) technique. A range of NADESs was evaluated to investigate the most suitable solvent system. The NADES synthesized from lactic acid and choline chloride provided the highest recovery of phenolics and flavonoids. Single-factor experiments were conducted to assess how individual process variables influence the recovery of phenolics and flavonoids. Key factors affecting extraction performance were assessed using the Plackett-Burman approach. Optimal conditions were established through the Box-Behnken Design model. The optimal extraction parameters for TPhC were determined as a solid-to-liquid ratio (SLR) of 1:59 g/mL, water content (WC) of 12 %, ultrasonic temperature of 43 °C, ultrasonic time of 6.88 min, and incubation time of 6.33 min. For TFlC, the optimal conditions were established at SLR of 1:67 g/mL, WC of 14 %, and enzyme activity of 1.28 U/g. Under these optimized conditions, UAE-EAE achieved the maximum recovery of total phenolic content (36.41 ± 2.50 mg GAE/g) and total flavonoid content (18.74 ± 1.28 mg QE/g). A second-order kinetic model was adopted to analyze the extraction mechanism. Structural and surface changes in the plant matrix before and after extraction were further investigated using scanning electron microscopy (SEM) and X-ray diffraction (XRD). This method demonstrates high efficiency and eco-friendliness in extracting plant-derived bioactive compounds using NADES-based UAE-EAE.

Abstract Image

绿色溶剂型超声-酶辅助萃取法提高越南香脂中酚类和类黄酮的回收率
研究了以天然深共熔溶剂(NADES)为基础的超声-酶辅助提取(UAE-EAE)技术对越南香薷中酚类和黄酮类物质的提取工艺。对一系列NADESs进行了评价,以确定最合适的溶剂体系。由乳酸和氯化胆碱合成的NADES对酚类和黄酮类化合物的回收率最高。通过单因素实验考察了各工艺变量对酚类和黄酮类化合物回收率的影响。使用Plackett-Burman方法评估影响提取性能的关键因素。通过Box-Behnken设计模型建立了最优条件。最佳提取条件为:料液比1:59 g/mL,水含量12%,超声温度43℃,超声时间6.88 min,超声培养时间6.33 min。最佳条件为SLR为1:67 g/mL, WC为14%,酶活性为1.28 U/g。在此优化条件下,UAE-EAE的总酚含量(36.41±2.50 mg QE/g)和总黄酮含量(18.74±1.28 mg QE/g)的回收率最高。采用二级动力学模型分析萃取机理。利用扫描电子显微镜(SEM)和x射线衍射仪(XRD)进一步研究了提取前后植物基质的结构和表面变化。该方法利用nades基UAE-EAE提取植物源性生物活性化合物具有较高的效率和环保性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Ultrasonics Sonochemistry
Ultrasonics Sonochemistry 化学-化学综合
CiteScore
15.80
自引率
11.90%
发文量
361
审稿时长
59 days
期刊介绍: Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信