Tan Phat Vo , Thi Hoang Trang Nguyen , Ha Bao Tran Nguyen , Hoang Nhan Nguyen , Nguyen Van Nhi Le , Minh Hoa Ha , Gia Bao Pham , Dinh Quan Nguyen
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引用次数: 0
Abstract
The research explored the extraction of phenolic and flavonoids from Elsholtzia ciliata (Vietnamese balm) using a natural deep eutectic solvent (NADES)-based ultrasonic-enzymatic-assisted extraction (UAE-EAE) technique. A range of NADESs was evaluated to investigate the most suitable solvent system. The NADES synthesized from lactic acid and choline chloride provided the highest recovery of phenolics and flavonoids. Single-factor experiments were conducted to assess how individual process variables influence the recovery of phenolics and flavonoids. Key factors affecting extraction performance were assessed using the Plackett-Burman approach. Optimal conditions were established through the Box-Behnken Design model. The optimal extraction parameters for TPhC were determined as a solid-to-liquid ratio (SLR) of 1:59 g/mL, water content (WC) of 12 %, ultrasonic temperature of 43 °C, ultrasonic time of 6.88 min, and incubation time of 6.33 min. For TFlC, the optimal conditions were established at SLR of 1:67 g/mL, WC of 14 %, and enzyme activity of 1.28 U/g. Under these optimized conditions, UAE-EAE achieved the maximum recovery of total phenolic content (36.41 ± 2.50 mg GAE/g) and total flavonoid content (18.74 ± 1.28 mg QE/g). A second-order kinetic model was adopted to analyze the extraction mechanism. Structural and surface changes in the plant matrix before and after extraction were further investigated using scanning electron microscopy (SEM) and X-ray diffraction (XRD). This method demonstrates high efficiency and eco-friendliness in extracting plant-derived bioactive compounds using NADES-based UAE-EAE.
期刊介绍:
Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels.
Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.