Assessment of anti-cancer activity of cyclovirobuxine D in nasopharyngeal carcinoma cells: Involvement of KIF23-mediated Akt/mTOR pathway

IF 3.2 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2025-09-01 DOI:10.1016/j.prp.2025.156204

Gang Li , Can Cui , Zheng Li

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引用次数: 0

Abstract

Cyclovirobuxine D (CVB-D) is a phytochemical ingredient that has potential antitumor effects in several types of cancer. However, its role and action mechanism in nasopharyngeal carcinoma (NPC) are still unclear. The present study found that CVB-D inhibited the viability of NPC cells, as well as induced NPC cell apoptosis. Kinesin family member 23 (KIF23) expression was inhibited by CVB-D treatment, and KIF23 knockdown exhibited viability-inhibitory and pro-apoptotic effects on NPC cells. Next, we demonstrated that overexpression of KIF23 prevented the inhibitory effect of CVB-D on cell viability, as well as its inductive effect on cell apoptosis. CVB-D treatment or KIF23 knockdown inhibited the Akt/mTOR pathway activation in NPC cells, with decreased expression levels of p-Akt and p-mTOR. Overexpression of KIF23 blocked the inhibitory effect of CVB-D on Akt/mTOR pathway activation. Additionally, Akt knockdown resisted KIF23-mediated antitumor effect of CVB-D. Our results indicated that CVB-D exerted viability-inhibitory and pro-apoptotic effects on NPC cells with the involvement of the KIF23/Akt/mTOR pathway. We provided experimental evidence for the antitumor potential and therapeutic implication of CVB-D in NPC.

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环virobuxine D在鼻咽癌细胞中的抗癌活性评估：kif23介导的Akt/mTOR通路的参与

环virobuxine D （CVB-D）是一种植物化学成分，对几种类型的癌症具有潜在的抗肿瘤作用。然而，其在鼻咽癌（NPC）中的作用和作用机制尚不清楚。本研究发现，CVB-D抑制鼻咽癌细胞活力，诱导鼻咽癌细胞凋亡。cbb - d处理可抑制Kinesin家族成员23 （KIF23）的表达，敲低KIF23对鼻咽癌细胞表现出抑制活力和促凋亡的作用。接下来，我们证明过表达KIF23可以阻止CVB-D对细胞活力的抑制作用，以及对细胞凋亡的诱导作用。CVB-D处理或敲低KIF23抑制鼻咽癌细胞中Akt/mTOR通路的激活，降低p-Akt和p-mTOR的表达水平。KIF23过表达阻断了CVB-D对Akt/mTOR通路激活的抑制作用。此外，Akt敲低抑制kif23介导的CVB-D抗肿瘤作用。我们的研究结果表明，CVB-D对鼻咽癌细胞具有活性抑制和促凋亡作用，其参与了KIF23/Akt/mTOR通路。我们为CVB-D在鼻咽癌中的抗肿瘤潜力和治疗意义提供了实验证据。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.