Illuminating diverse cellular contents with fluorogenic RNA aptamer-based biosensors: From standardization to innovation

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-08-27 DOI:10.1016/j.trac.2025.118436

Jianing Hou , Xinyu Liu , Chengwang Miao , Junyan Wang , Da Han

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Abstract

Capitalizing on the superior programmability of RNA for the precise targeting of diverse targets, fluorogenic RNA aptamer (FRAP)-based biosensors have become powerful tools for synthetic biology. By engineering FRAPs with recognition systems, these biosensors achieve remarkable specificity and sensitivity across detection targets spanning small molecules, proteins, and RNAs. Their unique capacity for real-time visualization of biomolecular dynamics renders them indispensable for living cell imaging applications. In this review, we discuss the generation process of FRAP-based biosensors, propose standardized methods for in vitro performance characterization, and provide a comprehensive analysis of their progress in living cell applications. Additionally, we analyzed strategies for improving biosensor performance in cellular environments. This review provides insights to accelerate the creation of FRAP-based biosensors, targeting diverse molecules, ultimately inspiring dynamic monitoring capabilities in living cell systems for decoding complex cellular responses.

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基于荧光RNA适配体的生物传感器：从标准化到创新

利用RNA优越的可编程性来精确靶向各种靶标，基于荧光RNA适体（FRAP）的生物传感器已经成为合成生物学的强大工具。通过设计带有识别系统的FRAPs，这些生物传感器在跨越小分子、蛋白质和rna的检测目标上实现了显著的特异性和灵敏度。它们独特的实时可视化生物分子动力学的能力使它们在活细胞成像应用中不可或缺。在本文中，我们讨论了基于frap的生物传感器的生成过程，提出了标准化的体外性能表征方法，并对其在活细胞中的应用进展进行了全面分析。此外，我们分析了在细胞环境中提高生物传感器性能的策略。这篇综述为加速基于frap的生物传感器的创建提供了见解，这些传感器针对不同的分子，最终激发了活细胞系统中解码复杂细胞反应的动态监测能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.