A U-box E3 ubiquitin ligase CmPUB15 targets CmMYB73 to regulate anthocyanin biosynthesis in response to low temperatures in chrysanthemum

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-09-02 DOI:10.1111/nph.70513

Zhiqiang Geng, Lijie Zhou, Yiguang Wang, Yuxi Wang, Zhenjie Shi, Daojin Sun, Yuhua He, Haibin Wang, Jiafu Jiang, Sumei Chen, Fadi Chen

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Abstract

During annual chrysanthemum production, low temperatures (10°C, the same below) can promote anthocyanin accumulation in petals. However, the specific mechanism behind this phenomenon remains unclear.
In this study, we identified CmMYB73, an SG22 R2R3-MYB transcription factor, as a negative regulator of anthocyanin biosynthesis in chrysanthemum. CmMYB73 responds to low temperatures in opposite ways at the transcriptional and protein levels.
Further investigations revealed that CmPUB15, a U-box E3 ubiquitin ligase, interacts with CmMYB73 and is significantly upregulated by low temperatures at the transcriptional level. CmMYB73 is ubiquitinated by CmPUB15 and subsequently degraded via the 26S proteasome pathway, leading to the accumulation of anthocyanin in chrysanthemum petals.
Our findings outline a novel pathway for anthocyanin biosynthesis under low temperatures and provide insights for molecular breeding efforts to enhance chrysanthemum flower color.

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一个U - box E3泛素连接酶CmPUB15靶向CmMYB73调控菊花对低温的花青素生物合成

在菊花生产过程中，低温（10℃，下同）可促进花瓣中花青素的积累。然而，这一现象背后的具体机制尚不清楚。在这项研究中，我们发现SG22 R2R3‐MYB转录因子CmMYB73是菊花花青素生物合成的负调控因子。CmMYB73在转录和蛋白质水平上以相反的方式响应低温。进一步的研究表明，U - box E3泛素连接酶CmPUB15与CmMYB73相互作用，并在转录水平上被低温显著上调。CmMYB73被CmPUB15泛素化，随后通过26S蛋白酶体途径降解，导致菊花花瓣中花青素的积累。我们的发现为低温下花青素的生物合成提供了一条新的途径，并为提高菊花颜色的分子育种工作提供了新的见解。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.