Exploration of photoelectrochemical immunosensing and quench-type signal amplification strategies based on oxygen-rich vacancy ternary FeCoCuOx nanostructures

IF 21.8 2区材料科学 Q1 MATERIALS SCIENCE, COMPOSITES

Advanced Composites and Hybrid Materials Pub Date : 2025-09-01 DOI:10.1007/s42114-025-01445-4

Junyong Mo, Haiyang Wang, Xiao Han, Dianping Tang, Xiwen Jiang

{"title":"Exploration of photoelectrochemical immunosensing and quench-type signal amplification strategies based on oxygen-rich vacancy ternary FeCoCuOx nanostructures","authors":"Junyong Mo, Haiyang Wang, Xiao Han, Dianping Tang, Xiwen Jiang","doi":"10.1007/s42114-025-01445-4","DOIUrl":null,"url":null,"abstract":"<div><p>Alpha-fetoprotein (AFP) is a key biomarker for the early diagnosis of hepatocellular carcinoma, while traditional detection methods often lack the required sensitivity and simplicity for the point-of-care testing. Herein, an innovative photoelectrochemical (PEC) immunoassay based on ternary FeCoCuO<sub>x</sub> nanostructures with abundant oxygen vacancies was developed for highly sensitive AFP detection. The engineered metal oxide provided an efficient photoactive interface, facilitating enhanced charge separation and light harvesting. In the sensing process, target AFP was specifically recognized by immobilized mAb<sub>1</sub>, followed by the binding of a signal probe (ALP-AuNP-pAb<sub>2</sub>: ALP and anti-AFP secondary antibody-labeled gold nanoparticle), which introduced alkaline phosphatase (ALP) into the system. ALP catalyzed the hydrolysis of 2-phospho-L-ascorbic acid (AAP) to generate ascorbic acid (AA), which acted as an electron donor under light irradiation and quenched the cathodic photocurrent by reacting with photoexcited holes. The PEC sensor achieved a low detection limit of 34.1 pg mL<sup>−1</sup> and a broad linear range from 0.05 to 50 ng mL<sup>−1</sup>. The superior performance was attributed to the synergistic enhancement from ALP-mediated signal amplification and the multimetallic oxide interface. This work demonstrates a robust and selective PEC platform with strong clinical potential for cancer biomarker detection.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":"8 5","pages":""},"PeriodicalIF":21.8000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42114-025-01445-4.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Composites and Hybrid Materials","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42114-025-01445-4","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, COMPOSITES","Score":null,"Total":0}

引用次数: 0

Abstract

Alpha-fetoprotein (AFP) is a key biomarker for the early diagnosis of hepatocellular carcinoma, while traditional detection methods often lack the required sensitivity and simplicity for the point-of-care testing. Herein, an innovative photoelectrochemical (PEC) immunoassay based on ternary FeCoCuO_x nanostructures with abundant oxygen vacancies was developed for highly sensitive AFP detection. The engineered metal oxide provided an efficient photoactive interface, facilitating enhanced charge separation and light harvesting. In the sensing process, target AFP was specifically recognized by immobilized mAb₁, followed by the binding of a signal probe (ALP-AuNP-pAb₂: ALP and anti-AFP secondary antibody-labeled gold nanoparticle), which introduced alkaline phosphatase (ALP) into the system. ALP catalyzed the hydrolysis of 2-phospho-L-ascorbic acid (AAP) to generate ascorbic acid (AA), which acted as an electron donor under light irradiation and quenched the cathodic photocurrent by reacting with photoexcited holes. The PEC sensor achieved a low detection limit of 34.1 pg mL⁻¹ and a broad linear range from 0.05 to 50 ng mL⁻¹. The superior performance was attributed to the synergistic enhancement from ALP-mediated signal amplification and the multimetallic oxide interface. This work demonstrates a robust and selective PEC platform with strong clinical potential for cancer biomarker detection.

Graphical Abstract

查看原文本刊更多论文

基于富氧空位三元feococox纳米结构的光电化学免疫传感和猝灭型信号放大策略探索

甲胎蛋白（AFP）是肝细胞癌早期诊断的关键生物标志物，而传统的检测方法往往缺乏所需的灵敏度和简便的即时检测。本文提出了一种基于富氧空位的feococox纳米结构的新型光电化学（PEC）免疫分析法，用于AFP的高灵敏度检测。工程金属氧化物提供了一个有效的光活性界面，促进了增强的电荷分离和光收集。在传感过程中，固定的mAb1特异性识别目标AFP，然后结合信号探针（ALP- aunp - pab2: ALP和抗AFP二抗标记的金纳米颗粒），将碱性磷酸酶（ALP）引入系统。ALP催化2-磷酸- l -抗坏血酸（AAP）水解生成抗坏血酸（AA），抗坏血酸在光照射下充当电子供体，与光激空穴反应猝灭阴极光电流。PEC传感器的检测限为34.1 pg mL−1，线性范围为0.05 ~ 50 ng mL−1。这种优异的性能是由于alp介导的信号放大和多金属氧化物界面的协同增强。这项工作证明了一个强大的、选择性的PEC平台，具有很强的癌症生物标志物检测的临床潜力。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advanced Composites and Hybrid Materials Multiple-

CiteScore

26.00

自引率

21.40%

发文量

185

期刊介绍： Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.