Assessing the antiviral potential of Bacillus subtilis and Pseudomonas putida strains against tomato yellow leaf curl virus: A focus on BU018 and ZA102

IF 4.5 Q1 PLANT SCIENCES
Farshad Hemmati, Fatemeh Norouzi, Keramatollah Izadpanah, Alireza Afsharifar
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Abstract

Plant viruses are responsible for approximately half of all epidemic plant diseases and cause significant damage to agricultural products. The resistance of plants developed using traditional or genetic engineering methods can be overcome by the genomic flexibility of viruses. On the other hand, no effective antiviral compounds are currently available for on-farm use against viruses. Multiple pieces of evidence indicate the potential of various chemical compounds and beneficial microorganisms to induce resistance against viruses in plants. Therefore, introducing resistance-inducing compounds may be a significant strategy for viral disease management. In the present study, tomato yellow leaf curl virus (TYLCV), one of the most damaging tomato viruses worldwide, was used as a model, and the effect of several bacteria isolated from the rhizosphere on its control was investigated. The bacteria were collected from various tomato fields in different provinces of Iran and purified and identified. Several properties of these bacteria, including IAA, EPS, and HCN production, were also examined. Based on these characteristics and the local lesion test on Nicotiana glutinosa plants, two strains of bacteria were selected for the experiments. Tomato seedlings at the three-to four-leaf stage were inoculated with TYLCV and then treated individually and in combination with the two strains, Bacillus subtilis strain BU018 and Pseudomonas putida strain ZA102. The bioactive compounds present in these two strains were measured using GC-MS. Changes in plant defense enzymes (POD, SOD, and CAT), transcription levels of several pathogenesis-related genes (NPR1, PR1, and PDF1.2), disease severity, virus concentration, and plant growth indices were investigated. The two strains resulted in 36.84 % and 21.05 % reductions in disease severity, respectively, compared to the control. These findings were confirmed by other analyses, including changes in the activity of plant defense enzymes, transcription levels of pathogenesis-related genes, virus concentration, and plant growth indices, indicating a reduction in disease severity by these two strains.
枯草芽孢杆菌和恶臭假单胞菌菌株对番茄黄卷叶病毒的抗病毒潜力评价——以BU018和ZA102为例
植物病毒约占所有植物流行病的一半,并对农产品造成重大损害。利用传统或基因工程方法开发的植物的抗性可以通过病毒基因组的灵活性来克服。另一方面,目前还没有有效的抗病毒化合物可用于农场对抗病毒。多项证据表明,各种化合物和有益微生物具有诱导植物对病毒产生抗性的潜力。因此,引入耐药诱导化合物可能是病毒性疾病管理的重要策略。本研究以世界上危害最大的番茄病毒之一番茄黄卷叶病毒(TYLCV)为模型,研究了从番茄根际分离的几种细菌对其的防治效果。这些细菌是从伊朗不同省份的番茄田收集的,并进行了纯化和鉴定。这些细菌的一些特性,包括IAA, EPS和HCN的生产,也进行了检查。在此基础上,通过对烟叶植物的局部损伤试验,选择了两株病原菌进行试验。用TYLCV接种3 ~ 4叶期番茄幼苗,分别与枯草芽孢杆菌BU018和恶臭假单胞菌ZA102联合处理。采用气相色谱-质谱法测定了两株菌株的活性成分。研究了植物防御酶(POD、SOD和CAT)、几种致病相关基因(NPR1、PR1和PDF1.2)的转录水平、疾病严重程度、病毒浓度和植物生长指标的变化。与对照相比,这两种菌株的疾病严重程度分别降低了36.84 %和21.05 %。这些发现得到了其他分析的证实,包括植物防御酶活性、致病相关基因转录水平、病毒浓度和植物生长指数的变化,表明这两种菌株降低了疾病严重程度。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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