{"title":"Identification of bile powder based on component data matrix and progressive matching strategy combined with UHPLC-QTOF-MS","authors":"Haonan Wu , Xianrui Wang , Minghua Li , Xiaohan Guo , Xianlong Cheng , Tian Yin , Wenguang Jing , Feng Wei","doi":"10.1016/j.bbrep.2025.102234","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Pig bile powder (PBP), chicken bile powder (CKBP), goat bile powder (GBP), bear bile powder (BBP), cow bile powder (CBP), rabbit bile powder (RBP) and snake bile powder (SBP) are commonly used bile powder medicinal materials in the clinic. Due to frequent confusion and fraudulent use, it is necessary to establish and enrich identification methods. In this paper, the component data matrix (CDM) contained common component matrix (CCM) and specificity component matrix (SCM), and progressive matching strategy (PMS) were innovatively proposed to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP.</div></div><div><h3>Methods</h3><div>The samples were analyzed by LC-MS to obtain MS data on chemical components. Then, the CCM and SCM of each bile powder was obtained by taking the intersection and de-intersection of MS data. Finally, the CCM and SCM were used for matching test samples to obtain matching value (MV). At the same time, the proprietary components were further explored.</div></div><div><h3>Results</h3><div>Based on CDM and PMS, the identification of PBP, CKBP, GBP, BBP, CBP, RBP and SBP can be quickly realized with MV ≥ 70 %. <strong>Conclusions</strong>: The CDM and PMS were efficient to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP, which has more specificity. It can enrich identification methods and strengthen quality assurance of bile powder medicinal materials.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102234"},"PeriodicalIF":2.2000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580825003218","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Pig bile powder (PBP), chicken bile powder (CKBP), goat bile powder (GBP), bear bile powder (BBP), cow bile powder (CBP), rabbit bile powder (RBP) and snake bile powder (SBP) are commonly used bile powder medicinal materials in the clinic. Due to frequent confusion and fraudulent use, it is necessary to establish and enrich identification methods. In this paper, the component data matrix (CDM) contained common component matrix (CCM) and specificity component matrix (SCM), and progressive matching strategy (PMS) were innovatively proposed to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP.
Methods
The samples were analyzed by LC-MS to obtain MS data on chemical components. Then, the CCM and SCM of each bile powder was obtained by taking the intersection and de-intersection of MS data. Finally, the CCM and SCM were used for matching test samples to obtain matching value (MV). At the same time, the proprietary components were further explored.
Results
Based on CDM and PMS, the identification of PBP, CKBP, GBP, BBP, CBP, RBP and SBP can be quickly realized with MV ≥ 70 %. Conclusions: The CDM and PMS were efficient to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP, which has more specificity. It can enrich identification methods and strengthen quality assurance of bile powder medicinal materials.
期刊介绍:
Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.