Zhen-Yu Qiang , Dong-Xing Guan , Jia-Lu Gao , Gang Li , Daniel Menezes-Blackburn , Anna Gunina , Yvan Capowiez , Lena Q. Ma
{"title":"Chemical imaging reveals phosphorus mobilization patterns in earthworm-engineered drilosphere","authors":"Zhen-Yu Qiang , Dong-Xing Guan , Jia-Lu Gao , Gang Li , Daniel Menezes-Blackburn , Anna Gunina , Yvan Capowiez , Lena Q. Ma","doi":"10.1016/j.geoderma.2025.117497","DOIUrl":null,"url":null,"abstract":"<div><div>Earthworms are soil ecosystem engineers who play a crucial role in phosphorus (P) cycling, encompassing inorganic P desorption and organic P mineralization. Although the role of earthworms in P mobilization is well documented, the spatial distribution of P mobilization processes and critical microbial species within the earthworm-engineered soil microhabitat, known as the drilosphere, remains to be determined. This study investigated P mobilization and redistribution in the drilosphere by <em>ex situ</em> chemical analyses, <em>in situ</em> soil zymography and diffusive gradients in thin-films imaging, and assessment of the microorganism communities. Endogeic earthworm species <em>Metaphire guillelmi</em> was incubated for 30 days in pots filled with soils Fluvisol or Acrisol with different total P contents (0.6 and 1.2 mg kg<sup>−1</sup>, respectively). Chemical analyses revealed that total P content in earthworm casts increased by 120 % in Fluvisol and 7.7 % in Acrisol. Available P content increased by 8.5 times in Fluvisol and 4.4 times in Acrisol in the drilosphere compared to bulk soil due to elevated acid/alkaline phosphatase activities and intensified Fe/Al-bound P desorption amongst others. Imaging identified co-existing and distinct hotspots for available P and acid phosphatase activity in soils surrounding the burrow walls, with hotspot proportions of 0.1 %‒3.1 % and 5.4 %‒7.5 % of the imaged areas. Earthworm activity increased the abundance of specific bacterial (<em>Aeromonas</em> and <em>Flavobacterium</em>) and fungal (<em>Scedosporium</em> and <em>Podospora</em>) taxa potentially contributing to P mobilization. The correlation between the distribution of metal-bound adsorption sites, phosphatase activity, and diffusion mechanisms contributed to the available P redistribution in the soil. These findings provide insights for developing soil management strategies that harness earthworm-mediated P cycling to optimize nutrient use efficiency and reduce external P inputs in sustainable agricultural systems.</div></div>","PeriodicalId":12511,"journal":{"name":"Geoderma","volume":"461 ","pages":"Article 117497"},"PeriodicalIF":6.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Geoderma","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0016706125003386","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Earthworms are soil ecosystem engineers who play a crucial role in phosphorus (P) cycling, encompassing inorganic P desorption and organic P mineralization. Although the role of earthworms in P mobilization is well documented, the spatial distribution of P mobilization processes and critical microbial species within the earthworm-engineered soil microhabitat, known as the drilosphere, remains to be determined. This study investigated P mobilization and redistribution in the drilosphere by ex situ chemical analyses, in situ soil zymography and diffusive gradients in thin-films imaging, and assessment of the microorganism communities. Endogeic earthworm species Metaphire guillelmi was incubated for 30 days in pots filled with soils Fluvisol or Acrisol with different total P contents (0.6 and 1.2 mg kg−1, respectively). Chemical analyses revealed that total P content in earthworm casts increased by 120 % in Fluvisol and 7.7 % in Acrisol. Available P content increased by 8.5 times in Fluvisol and 4.4 times in Acrisol in the drilosphere compared to bulk soil due to elevated acid/alkaline phosphatase activities and intensified Fe/Al-bound P desorption amongst others. Imaging identified co-existing and distinct hotspots for available P and acid phosphatase activity in soils surrounding the burrow walls, with hotspot proportions of 0.1 %‒3.1 % and 5.4 %‒7.5 % of the imaged areas. Earthworm activity increased the abundance of specific bacterial (Aeromonas and Flavobacterium) and fungal (Scedosporium and Podospora) taxa potentially contributing to P mobilization. The correlation between the distribution of metal-bound adsorption sites, phosphatase activity, and diffusion mechanisms contributed to the available P redistribution in the soil. These findings provide insights for developing soil management strategies that harness earthworm-mediated P cycling to optimize nutrient use efficiency and reduce external P inputs in sustainable agricultural systems.
期刊介绍:
Geoderma - the global journal of soil science - welcomes authors, readers and soil research from all parts of the world, encourages worldwide soil studies, and embraces all aspects of soil science and its associated pedagogy. The journal particularly welcomes interdisciplinary work focusing on dynamic soil processes and functions across space and time.