Comparative profiling of splenic phospholipid and sphingolipid during acute infection

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-01 DOI:10.1016/j.micpath.2025.108015

Tao Liu , Hong Jia , Hui Dong , Chaofei Cheng , Tao Zhang , Xinghui Zhao , Zhanzhong Zhao

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Abstract

Lipid profile of spleen and bursa of Fabricius (BF) during acute infection remains unknown. Acute infection models of porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and Eimeria tenella (ET) were developed, and spleen samples with African swine fever virus (ASFV) or not were collected. Spleen and BF were examined and characteristic microscopic lesions were observed. Samples were analyzed using a semi-quantitative, untargeted method based on liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS-MS). After statistical analysis, potential biomarkers for PRRSV [16:0 20:5 phosphatidylcholine (16:0 20:5 PC), 18:1 lysophosphatidylcholine (18:1 LPC)], for PEDV [16:0 20:4 phosphatidylinositol (16:0 20:4 PI), 18:0 18:2 phosphatidylserine (18:0 18:2 PS), 16:0 lysophosphatidylserine (16:0 LysoPS), 18:0 LysoPS, d18:1 16:0 sphingomyelin (d18:1 16:0 SM)], for ASFV [16:0 18:0 PC, 16:0 20:4 PC, 18:0 20:4 PC, O-16:0 20:4 PC, O-18:1 20:4 PC, 16:0 18:2 phosphatidylethanolamine (16:0 18:2 PE), 16:0 18:1 PE, 18:0 20:4 PE, 16:0 18:2 PI, 18:0 18:1 PS], for ET with spleen [16:0 20:3 PC, 18:0 18:2 PC, 18:0 18:1 PI, 18:0 20:4 PI, 18:0 22:6 PI, d18:1 16:0 SM, d18:1 22:1 SM], for ET with BF [16:0 18:2 PI, 16:0 18:1 PI, 18:0 18:2 PI, 18:0 20:4 PI, 16:0 18:2 PS, 16:0 18:1 PS, 18:0 18:2 PS, 18:0 20:4 PS, 18:0 22:6 PS, 18:0 LysoPS, 20:4 arachidonic acid (20:4 AA), 22:6 docosahexaenoic acid (22:6 DHA)] were analyzed. Overall, lipid profiling of spleen and BF was demonstrated, contributing to understand the lipid biology of lymphoid organs and intervene acute infection.

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急性感染时脾磷脂和鞘磷脂的比较分析

急性感染时脾脏和法氏囊（BF）的脂质谱尚不清楚。建立猪繁殖与呼吸综合征病毒（PRRSV）、猪流行性腹泻病毒（PEDV）和细嫩艾美耳虫（ET）急性感染模型，采集感染和未感染非洲猪瘟病毒（ASFV）的脾脏标本。检查脾脏和BF，观察特征性显微镜病变。样品采用液相色谱-电喷雾串联质谱（LC-MS-MS）半定量、非靶向方法进行分析。统计分析后,PRRSV的潜在生物标志物(16:0二十5磷脂酰胆碱(16:0二十5个人电脑)18:1 lysophosphatidylcholine (18:1 LPC)],为PEDV(16:0 20:4磷脂酰肌醇(16:0 20:4π)18:0 18:2磷脂酰丝氨酸(18:0 18:2 PS), 16:0 lysophosphatidylserine (16:0 LysoPS), 18:0 LysoPS, d18:1 16:0鞘磷脂(d18:1 16:0 SM)], ASFV [16:0 18:0 PC, 16:0 20:4 PC, 18:0 20:4 PC, O-16:0 20:4 PC, O-18:1 20:4 PC, 16:0 18:2磷脂酰乙醇胺(16:0 18:2 PE), 16:0 18:1 PE、18:0 20:4 PE、16:0十八2π,18:0 18:1 PS),与脾等(16:0 20:3 PC, 18:0 18:2 PC, 18:0 18:1π,18:0 20:4π,18:0 22:6π,d18:1 16:0 SM, d18:1 22:1 SM),与男朋友ET(16:0十八2π,16:0 18:1π,18:0十八2π,18:0 20:4π,16:0 18:2 PS, 16:0 18:1 PS, 18:0 18:2 PS, 18:0 20:4 PS, 18:0 22:6 PS, 18:0 LysoPS, 20:4花生四烯酸(AA 20:4) 22:6二十二碳六烯酸(DHA 22:6)]进行了分析。总之，脾脏和BF的脂质谱分析，有助于了解淋巴器官的脂质生物学和干预急性感染。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)