Acaricidal activity of green nanoparticles (TiO2) against Haemaphysalis bispinosa and Rhipicephalus (Boophilus) microplus

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-08-29 DOI:10.1016/j.vetpar.2025.110590

Sandhiya Chandran , Banumathi Balan , Sibiya Ashokkumar , Shine Kadaikunnan , Marcello Nicoletti , Marimuthu Govindarajan , Vaseeharan Baskaralingam

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Abstract

In the present work, the in vitro efficacy of titanium isopropoxide, Cassia auriculata flower extract and C. auriculata-mediated titanium nanoparticles (Ca-TiO₂ NPs) as an acaricidal drug against Rhipicephalus (Boophilus) microplus (larvae, nymph and adult) and Haemaphysalis bispinosa (adult) was evaluated. The synthesized nanomaterial was then characterized through UV, XRD, FTIR, Zeta potential and HR-TEM techniques. UV-Vis spectroscopy showed an absorption peak at 289 nm. Bragg’s peaks corresponding to 1 0 1, 0 0 4, 2 0 0, 1 0 5, 2 1 1, 2 0 4, 1 1 0, 2 2 0, and 2 1 5 planes in XRD analysis confirm the crystalline structure of Ca-TiO₂ nanoparticles. FTIR analysis identified the functional groups of Ca-TiO₂ NPs in the band range of 3316–573 cm⁻¹. Zeta potential represents the surface charge and stability of Ca-TiO₂ nanoparticles. HR-TEM analysis showed the hexagonal shape of Ca-TiO₂ nanoparticles with size distribution in the range of 70–140 nm. All life stages were affected in R. (B.) microplus: mortality was induced in larvae, nymphs and adults after treatment with Ca-TiO₂ NPs, but only adults from H. bispinosa died. At the concentration of 100 µg/mL, the adult mortality rates of R. (B.) microplus and H. bispinosa were noted to be 74 % and 68 %, respectively, after treatment with Ca-TiO₂ NPs. R. (B.) microplus nymph and larva mortality was 90 % at 100 µg/mL and 100 % at 16 µg/mL. On the other hand, the C. auriculata flower extract gave 28 % adult mortality against R. (B.) microplus and 27 % for H. bispinosa at the highest concentration of 100 µg/mL. Larval and nymph mortality rates of R. (B.) microplus were 68.8 % (16 µg/mL) and 44 % (100 µg/mL). The light microscopy of embryos from R. (B.) microplus distinctly shows that embryonic development is arrested. Ca-TiO₂ nanoparticles showed potential acaricidal activity and egg hatchability inhibition activity compared to C. auriculata flower extract and titanium isopropoxide.

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绿色纳米粒子（TiO2）对双皮血蜱和微猪头虫的杀螨活性研究

本研究对异丙醇钛、木耳花提取物和木耳介导的纳米钛（Ca-TiO2 NPs）作为杀螨药物对微头虫（幼虫、若虫和成虫）和双皮血蜱（成虫）的体外药效进行了评价。然后通过UV、XRD、FTIR、Zeta电位和HR-TEM等技术对合成的纳米材料进行了表征。紫外可见光谱显示其吸收峰位于289 nm处。在XRD分析中，对应于1 0 1、0 0 4、2 0 0、1 0 5、2 1 1、2 0 4、1 1 0、2 2 0和2 1 5平面的Bragg峰证实了Ca-TiO2纳米粒子的晶体结构。FTIR分析确定了Ca-TiO2 NPs在3316-573 cm−1波段的官能团。Zeta电位表征了Ca-TiO2纳米粒子的表面电荷和稳定性。hrtem分析表明，Ca-TiO2纳米粒子呈六角形，粒径分布在70 ~ 140 nm之间。Ca-TiO2 NPs处理后，微纹夜蛾的幼虫、若虫和成虫均有死亡，但只有成虫死亡。在浓度为100 µg/mL的浓度下，Ca-TiO2 NPs处理后，微加褐螺旋藻和双色褐螺旋藻的成虫死亡率分别为74 %和68 %。在100 µg/mL和16 µg/mL处理下，微加线虫若虫和幼虫的死亡率分别为90 %和100 %。另一方面，在最高浓度为100 µg/mL时，木耳花提取物对小弧菌的成虫死亡率为28% %，对双色木耳的成虫死亡率为27% %。微夜蛾幼虫和若虫死亡率分别为68.8 %（16 µg/mL）和44 %（100 µg/mL）。胚胎的光镜观察清楚地显示胚胎发育被阻滞。与木耳花提取物和异丙醇钛相比，Ca-TiO2纳米粒子具有潜在的杀螨活性和抑制卵孵化的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.