Proteomics identifies critical hemolymph proteins for extreme temperature adaptation in Hyalomma asiaticum

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-08-28 DOI:10.1016/j.vetpar.2025.110587

Han Wang , Panyi Yang , Abolfazl Masoudi , Haili Yun , Yue Gao , Ziheng Zhao , Zhijun Yu , Hui Wang

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Abstract

Hyalomma asiaticum can survive in extreme environments including the semi-arid zones and deserts. Investigating the molecular mechanisms of the tolerance of ticks to extreme temperatures could help tick control by interfering with their temperature tolerance. A comprehensive analysis of the regulatory mechanisms of proteins in the hemolymph is essential to unraveling the survival strategies of ticks in harsh environments. In this study, a quantitative proteomics analysis was performed by data independent acquisition (DIA) using hemolymph of ticks exposed to extreme temperatures. Proteins involved in energy metabolism, cuticle protection, and cell cycle changed under extreme temperatures. Further analysis using RNA interference (RNAi) revealed that when a secreted glycine-rich protein (GRP) was knocked down, the survival probability of ticks at −10°C was 20 %, and when glucosamine-phosphate N-acetyltransferase (GNPNAT) and V-atpase subunit H (V-ATPase) were knocked down, the survival probability of ticks at 46°C were 15 % and 20 %, respectively. The results showed that maintaining lipid homeostasis, ensuring energy supply and enhancing ATPase catalytic efficiency were the most important means for ticks to survive at extreme temperatures. This may contribute to our understanding of temperature adaptation mechanisms in ticks.

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蛋白质组学鉴定了亚洲透明瘤极端温度适应的关键血淋巴蛋白

亚洲透明瘤可以在极端环境中生存，包括半干旱地区和沙漠。研究蜱对极端温度耐受的分子机制可以通过干扰它们的温度耐受性来帮助控制蜱。全面分析血淋巴中蛋白质的调节机制对于揭示蜱在恶劣环境中的生存策略至关重要。本研究采用数据独立采集（data independent acquisition， DIA）方法，对暴露在极端温度下的蜱血淋巴进行了定量蛋白质组学分析。参与能量代谢、角质层保护和细胞周期的蛋白质在极端温度下发生了变化。通过RNA干扰（RNAi）进一步分析发现，敲除富甘氨酸分泌蛋白（GRP）后，蜱在- 10℃下的生存概率为20 %；敲除磷酸氨基葡萄糖n -乙酰转移酶（GNPNAT）和v -atp酶亚基H （v -atp酶）后，蜱在46℃下的生存概率分别为15 %和20 %。结果表明，维持脂质稳态、保证能量供应和提高atp酶催化效率是蜱在极端温度下生存的最重要手段。这可能有助于我们理解蜱虫的温度适应机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.