Screening of essential oils with acaricidal activity against the poultry red mite (Dermanyssus gallinae) and analysis of active components

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-08-29 DOI:10.1016/j.vetpar.2025.110589

Jing Liu , Shuo Yin , Zhonghao Wang , Jianhua Qin , Dehe Wang , Hui Chen , Lijun Xu , Chuanwen Wang

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Abstract

Dermanyssus gallinae is a common ectoparasite in the laying hen industry, damaging chicken health by sucking blood. With mites developing acaricide resistance, there's an urgent need for alternatives. In this study, we evaluated the efficacy of 34 essential oils (EOs) on D. gallinae through contact and fumigation toxicity tests. Results from contact efficacy studies showed that clove basil, cinnamon, thyme, wintergreen, oregano, palmarosa, lemongrass and geranium EOs possessed significant acaricidal activity with LC₅₀ values ranging from 0.3 to 1.0 mg/cm², among which cinnamon was the most toxic. In the fumigation assay, oregano, lemongrass, palmarosa, clove basil, thyme and cinnamon both in the closed and open systems had a significantly higher vapour efficacy than others after 72 h (P < 0.01), with mite mortality of 100.0 %. Cinnamon possessed the highest residual toxicity, with its effectiveness lasting up to 3 days. Clove basil, cinnamon, geranium, palmarosa, oregano and thyme exhibited effective ovicidal activity, with egg hatchability of 1.7 ± 2.9, 13.3 ± 7.6, 15.0 ± 13.2, 0.0 ± 0.0, 16.67 ± 10.4, and 18.33 ± 2.9 %, respectively. Among them, palmarosa achieved the most significant ovicidal activity. GC-MS analysis of the major compounds in the eight EOs found eugenol (77.9 %) was dominant in clove basil; trans-cinnamaldehyde (80.1 %) in cinnamon; cephrol (20.9 %) and citronellyl formate (11.4 %) in geranium; geranaldehydeial (39.7 %) and neral (36.9 %) in lemongrass; carvacrol (55.8 %) in oregano; thymol (30.3 %) in thyme; geraniol (41.0 %) and neryl alcohol (28.3 %) in palmarosa; and methyl salicylate (99.4 %) in wintergreen. These results suggest that the eight most toxic EOs may serve as effective and environmentally friendly alternatives to conventional acaricides.

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对家禽红螨有杀螨活性的精油筛选及活性成分分析

鸡皮虫是蛋鸡行业中常见的一种体外寄生虫，通过吸血危害鸡的健康。随着螨虫对杀螨剂产生抗药性，我们迫切需要替代品。本研究通过接触毒性试验和熏蒸毒性试验，评价了34种精油对鸡瘿蝇的毒力。接触药效试验结果表明，罗勒、桂皮、百里香、冬青、牛至、棕榈叶、柠檬草和天竺葵的LC50值在0.3 ~ 1.0 mg/cm2之间，其中桂皮的毒性最大。熏蒸72 h后，牛至叶、柠檬草、棕榈叶、罗勒叶、百里香和肉桂在封闭和开放体系中的蒸效均显著高于其他体系（P <； 0.01），螨死亡率为100.0 %。肉桂具有最高的残余毒性，其有效性持续3天。丁香罗勒、肉桂、天竺葵、palmarosa、牛至和百里香的活动有效的杀卵,卵孵化率为1.7 ± 2.9,13.3 ± 7.6,15.0 ± 13.2,0.0 ± 0.0,16.67 ± 10.4,和18.33 ±2.9  %,分别。其中，棕榈草具有最显著的杀卵活性。GC-MS分析发现，丁香酚（77.9% %）在罗勒中占主导地位；肉桂中的反式肉桂醛（80.1 %）；天竺葵中樟脑（20.9 %）和甲酸香茅酯（11.4 %）；香茅中香叶醛（39.7 %）和木犀醛（36.9 %）；牛至中的香芹酚（55.8% %）；百里香中的百里酚（30.3 %）；香叶醇（41.0 %）和橙醇（28.3 %）；冬青中水杨酸甲酯含量为99.4 %。这些结果表明，八种毒性最强的环氧乙烷可以作为传统杀螨剂的有效和环保替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.