Decoding the Chemodiversity blueprint: Chromosome-scale genome assembly unveils photosynthesis-terpenoid coordination in Cinnamomum burmanni through genomic and miRNA regulatory networks

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-08-28 DOI:10.1016/j.plantsci.2025.112733

Chen Hou, Yanling Cai, Jun Yao, Peiwu Xie, Boxiang He, Huimign Lian, Yingli Wang, Yonglin Zhong, Bing Li, Minghuai Wang, Qian Zhang

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引用次数: 0

Abstract

The intricate interplay between photosynthetic efficiency and terpenoid biosynthesis in plants remains a pivotal yet underexplored area in secondary metabolism research. This study elucidates the physiological and molecular mechanisms underlying this synergy in Cinnamomum burmanni, a chemically diverse Lauraceae species, through a multi-omics approach. A high-quality chromosome-level genome of C. burmanni (1.14 Gb, scaffold N50: 94.90 Mb) was assembled, and evolutionary analyses revealed species-specific gene family expansions, particularly in mono-, sesqui-, and diterpenoid biosynthesis pathways. Comparative analyses of photosynthetic traits across chemotypes demonstrated that the borneol-type exhibits superior photosynthetic capacity, characterized by elevated net photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO₂, carboxylation efficiency and non-photochemical quenching. The phenotype is linked to upregulated chlorophyll metabolism, carotenoid biosynthesis regulators, and enhanced light-harvesting complex and photosystem components, optimizing light energy conversion. Mechanistically, photosynthetic activity modulates precursor flux into terpenoid pathways by regulating rate-limiting enzymes. Additionally, lineage-specific expansions of terpene synthase and isopentenyl diphosphate synthase gene families underpin specialized terpenoid production. A post-transcriptional regulatory network involving 14 miRNAs (e.g., miR396, miR2950) was identified, coordinately targeting 11 key genes in both photosynthesis and terpenoid synthesis, suggesting a dual role in metabolic fine-tuning. This work advances understanding of the evolutionary and physiological integration of photosynthesis and secondary metabolism in aromatic plants, offering a genomic foundation for biotechnological applications in metabolite synthesis and chemotype breeding.

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解码化学多样性蓝图：通过基因组和miRNA调节网络，染色体尺度的基因组组装揭示了缅甸肉桂光合作用-萜类化合物的协调

植物光合效率与萜类生物合成之间复杂的相互作用是次生代谢研究中一个关键但尚未得到充分研究的领域。本研究通过多组学方法阐明了樟科化学多样性物种肉桂（Cinnamomum burmanni）协同作用的生理和分子机制。一个高质量的burmanni染色体水平基因组（1.14 Gb, scaffold N50: 94.90 Mb）被组装，进化分析揭示了物种特异性基因家族的扩展，特别是在单、倍半和二萜类生物合成途径中。不同化学型光合特性的比较分析表明，冰片型表现出更高的光合能力，其特征是净光合速率、蒸腾速率、气孔导度、胞间CO2、羧化效率和非光化学猝灭。该表型与叶绿素代谢上调、类胡萝卜素生物合成调节因子、光收集复合物和光系统成分增强、优化光能转换有关。从机制上讲，光合活性通过调节限速酶来调节进入萜类化合物途径的前体通量。此外，萜烯合成酶和异戊烯二磷酸合成酶基因家族的谱系特异性扩增支撑了专门的萜烯生产。发现了一个包含14个mirna（如miR396、miR2950）的转录后调控网络，协调靶向光合作用和萜类化合物合成中的11个关键基因，表明其在代谢微调中具有双重作用。这项工作促进了对芳香植物光合作用和次生代谢的进化和生理整合的理解，为代谢物合成和化学型育种的生物技术应用提供了基因组基础。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.