Claudine Uwamahoro , Jae-Hwan Jo , Seung-Ik Jang , Eun-Ju Jung , Woo-Jin Lee , Jeong-Won Bae , Daehyun Kim , Junkoo Yi , Sangsu Shin , Joonho Moon , Woo-Sung Kwon
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引用次数: 0
Abstract
The potential toxicity of abamectin (ABM), a macrocyclic lactone insecticide widely used in agriculture, in non-target organisms has raised concerns. Therefore, this study aimed to investigate the effects of ABM on sperm function, focusing on motility, kinematics, ATP levels, viability, protein kinase A (PKA) activity, and tyrosine phosphorylation. Boar spermatozoa were incubated with various ABM concentrations (0, 1, 5, 10, 15, 25, 50, and 100 µM) under capacitating conditions and assessed through computer-assisted sperm analysis, Hoechst 33258/chlortetracycline staining, ATP and viability assays, and Western blotting. The results revealed a dose-dependent decline in sperm motility and ATP levels starting at 10 µM. Sperm capacitation was significantly altered at concentrations of at least 25 µM, with an increase in capacitated spermatozoa and a decrease in non-capacitated spermatozoa. However, overall cell viability remained unaffected. Western blot analysis showed abnormal alterations in PKA substrates and tyrosine-phosphorylated proteins. These findings suggest ABM disrupts sperm function and key fertilization pathways, indicating potential risks to male fertility.
期刊介绍:
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.