Eliminate drug-resistant bacterial infection and accelerate cutaneous wound repair by antimicrobial, angiogenic, and immunomodulating microneedles

IF 7.4 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Science China Materials Pub Date : 2025-07-25 DOI:10.1007/s40843-025-3477-2

Shuo Wang (, ), Jiaheng Liang (, ), Mengjie Sun (, ), Jin Chai (, ), Weihao Zhao (, ), Yibo Yan (, ), Peng Li (, )

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引用次数: 0

Abstract

Bacterial infection inevitably disrupts wound repair processes, including the inflammatory response and angiogenesis, thus impairing healing. Emerging antibiotic resistance makes drug-resistant bacterial wound infection a serious challenge in clinical practice. The efficacy of conventional wound dressings for therapeutic delivery is constrained by the barrier effects of skin. Herein, we present a novel strategy using a dissolving microneedle (MN) system for transderamlly delivering ε-poly-L-lysine (EPL)/hyaluronic acid (HA) nanoparticles (EH NPs) to effectively eliminate drug-resistant bacteria infection and accelerate wound healing. The electrostatic co-assembled EH NPs improved the bioactivities of two ingredients due to enhanced cell phagocytosis, enabling combinational antimicrobial, angiogenic, and anti-inflammatory abilities. In vitro studies indicated that this MN system achieved effective killing of Methicillin-resistant Staphylococcus aureus (>99.9%), upregulating endogenous nitric oxide release and CD31 expression in human vascular endothelial cells, and promoting the polarization of macrophages from Ml to M2. In a drug-resistant bacteria-infected skin wound mouse model, this MN system effectively promoted granulation tissue formation and collagen deposition by enhancing angiogenesis and reducing the inflammatory response, thereby significantly accelerating wound healing.

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通过抗菌、血管生成和免疫调节微针消除耐药细菌感染，加速皮肤伤口修复

细菌感染不可避免地破坏伤口修复过程，包括炎症反应和血管生成，从而损害愈合。新出现的抗生素耐药性使耐药细菌性伤口感染成为临床实践中的一个严峻挑战。传统伤口敷料的治疗效果受到皮肤屏障作用的限制。在此，我们提出了一种新的策略，利用溶解性微针（MN）系统经皮递送ε-聚l -赖氨酸(EPL)/透明质酸（HA）纳米颗粒（EH NPs），有效消除耐药细菌感染，加速伤口愈合。静电共组装的EH NPs通过增强细胞吞噬作用提高了两种成分的生物活性，实现了抗菌、血管生成和抗炎的综合能力。体外研究表明，该MN系统能有效杀伤耐甲氧西林金黄色葡萄球菌（>99.9%），上调人血管内皮细胞内源性一氧化氮释放和CD31表达，促进巨噬细胞从Ml向M2极化。在耐药细菌感染的皮肤创面小鼠模型中，该MN系统通过促进血管生成和减少炎症反应，有效促进肉芽组织形成和胶原沉积，从而显著加速创面愈合。

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来源期刊

Science China Materials Materials Science-General Materials Science

CiteScore

11.40

自引率

7.40%

发文量

949

期刊介绍： Science China Materials (SCM) is a globally peer-reviewed journal that covers all facets of materials science. It is supervised by the Chinese Academy of Sciences and co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China. The journal is jointly published monthly in both printed and electronic forms by Science China Press and Springer. The aim of SCM is to encourage communication of high-quality, innovative research results at the cutting-edge interface of materials science with chemistry, physics, biology, and engineering. It focuses on breakthroughs from around the world and aims to become a world-leading academic journal for materials science.