Surface-Enhanced Raman Spectroscopy for the Characterization of Filtrate Portions of Blood Serum Samples of Malaria Patients Using 30 kDa Filtration Devices

IF 4.3 4区物理与天体物理 Q2 CHEMISTRY, PHYSICAL

Plasmonics Pub Date : 2025-01-11 DOI:10.1007/s11468-025-02760-8

Rafia Atta, Zainub Shoukat, Haq Nawaz, Muhammad Irfan Majeed, Nosheen Rashid, Norah A. Albekairi, Abdulrahman Alshammari, Aleena Shahzadi, Sonia Yaseen, Iqra Arshad, Mariam Shakeel, Taram Nayab, Mahrosh Javed

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引用次数: 0

Abstract

Raman spectroscopy is a nondestructive tool for initial diagnosis of different diseases by using bodily fluids, including blood serum samples which having high and low molecular weight fractions (LMWF and HMWF) of proteins. The analysis of disease biomarkers is difficult because they mainly consist of LMWF of proteins which is suppressed by HMWF of proteins like albumin and globulin. The main focus of this study is to overcome this problem; 30 kDa centrifugal filtration devices were utilized to separate the filtrate portions of serum of malaria patients which have the marker proteins of malaria with size less than 30 kDa. For this purpose, surface-enhanced Raman spectroscopy (SERS), using silver nanoparticles (Ag-NPs) as SERS substrate, is used to examine biochemical variations occurring in the filtrate portions of serum of malaria patients to establish an easy and reliable method for diagnosis of malaria. SERS helped in distinguishing serum of healthy individuals from malarial patients on the basis of their characteristic SERS spectral features. The chemometric tool such as principal component analysis (PCA) is used for the analysis of SERS spectra of various classes of 30 kDa filtrate samples. PCA helped in the qualitative analysis of SERS spectra of filtrate portions of malaria and healthy filtrate.

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使用30 kDa过滤装置的表面增强拉曼光谱用于疟疾患者血清样品滤液部分的表征

拉曼光谱是一种非破坏性的工具，通过使用体液，包括具有高分子量和低分子量蛋白质（LMWF和HMWF）的血清样本，对不同疾病进行初步诊断。疾病生物标志物的分析是困难的，因为它们主要由蛋白质的低分子量f组成，这些蛋白质被白蛋白和球蛋白等蛋白质的低分子量f抑制。本研究的主要重点是克服这一问题；采用30kda离心过滤装置分离疟疾患者血清滤液中大小小于30kda的疟疾标记蛋白。为此，采用纳米银粒子（Ag-NPs）作为SERS底物，利用表面增强拉曼光谱（SERS）检测疟疾患者血清滤液部分发生的生化变化，从而建立一种简便可靠的疟疾诊断方法。基于个体的SERS谱特征，SERS有助于区分健康个体和疟疾患者的血清。主成分分析（PCA）等化学计量学工具用于分析30 kDa滤液样品的各种类别的SERS光谱。PCA有助于疟疾滤液和健康滤液部分SERS谱的定性分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasmonics 工程技术-材料科学：综合

CiteScore

5.90

自引率

6.70%

发文量

164

审稿时长

2.1 months

期刊介绍： Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons. Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.