Bright Thermo-resilient and Promiscuous Zombie Protein for Lighting Applications

IF 8.7 1区化学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Materials Letters Pub Date : 2025-07-25 DOI:10.1021/acsmaterialslett.5c00653

Marta Patrian, Marco Hasler, Jesús A. Banda-Vázquez, Evgenia Borisova, Juan Pablo Fuenzalida Werner and Rubén D. Costa*,

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引用次数: 0

Abstract

Proteins are at the forefront of materials science, with implementations in optical, electrical, and structural materials for transformative and sustainable technologies. Within the biohybrid light-emitting diode (BioHLED) concept, replacing toxic and/or rare photon filters with classical β-barrel fluorescent proteins (FPs) that must withstand irradiation, temperature, oxidation, and dehydration stress, the question if FPs from extremophiles and/or living fossils might be better for lighting applications arises. We addressed this by introducing a thermostable prokaryotic FP, whose inherent promiscuity enables the design of tunable emitting proteins. Three milestones were reached: (i) a comprehensive phylogeny of phycobiliproteins from a large data set (182 proteins from 29 thermophiles) to identify the most versatile zombie-like phycobiliprotein (highly ancestral character), (ii) heterologous expression of this phycobiliprotein (SPritZ) in Escherichia coli and further enhancement via rational mutagenesis into a brighter and more thermal-resilient variant (eSPritZ), and (iii) 2.5-fold stable BioHLEDs comparing SPritZ vs eSPritZ in hydroxypropyl cellulose coatings.

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明亮的热弹性和混杂的僵尸蛋白照明应用

蛋白质处于材料科学的前沿，在光学、电气和结构材料中实现了变革和可持续技术。在生物混合发光二极管（BioHLED）概念中，用经典的β-桶状荧光蛋白（FPs）取代有毒和/或稀有光子滤光片，必须承受辐射，温度，氧化和脱水压力，问题是极端微生物和/或活化石的FPs是否更适合照明应用。我们通过引入耐热的原核FP来解决这个问题，其固有的混杂性使设计可调节的发射蛋白成为可能。达成了三个里程碑：(i)从一个大型数据集（来自29种嗜热菌的182个蛋白质）中对藻胆蛋白进行全面的系统发育，以确定最通用的僵尸样藻胆蛋白（高度祖先特征）；（ii）在大肠杆菌中异种表达这种藻胆蛋白（SPritZ），并通过合理的突变进一步增强为更亮、更耐热的变体（eSPritZ）；（iii）在羟丙基纤维素涂层中比较SPritZ和eSPritZ的2.5倍稳定性的BioHLEDs。

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来源期刊

ACS Materials Letters MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.60

自引率

3.50%

发文量

261

期刊介绍： ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.