Cellular Uptake and Antioxidant Activity of H2S-Releasing Tetrapeptide Supramolecular Polymer Nanostructures

IF 8.7 1区化学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Materials Letters Pub Date : 2025-08-07 DOI:10.1021/acsmaterialslett.5c00921

Zhao Li, Bushra Tousian, Michal Zaiden, Ishani Sarkar, Clark Vu, Yin Wang*, Ronit Bitton* and John B. Matson*,

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引用次数: 0

Abstract

Self-assembled peptide–H₂S donor conjugates (PHDCs) can deliver hydrogen sulfide in vitro and in vivo, yet the link between the supramolecular nanostructure morphology and cellular uptake remains unclear. Herein, we designed constitutionally isomeric PHDCs that self-assembled in aqueous solution into either nanoribbons, nanofibers, or nanobelts with various dimensions based on cryogenic transmission electron microscopy and small-angle X-ray scattering. Nile-red loaded PHDCs showed morphology-dependent uptake by H9C2 cells based on fluorescence microscopy combined with flow cytometry and confocal imaging, where narrow, helically twisted nanoribbons entered most efficiently. All PHDCs released H₂S at similar rates, but the amount of H₂S released inside the cells depended on the internalization ability of each PHDC. Consistent with these results, the narrow twisted nanoribbons afforded the greatest protection against H₂O₂-induced oxidative stress. Overall, this study highlights how subtle molecular-level changes can influence nanostructure formation in supramolecular assemblies and ultimately affect their cellular uptake and biological activities.

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释放硫化氢的四肽超分子聚合物纳米结构的细胞摄取和抗氧化活性

自组装肽- h2s供体偶联物（PHDCs）可以在体外和体内传递硫化氢，但其超分子纳米结构形态与细胞摄取之间的联系尚不清楚。基于低温透射电子显微镜和小角度x射线散射，我们设计了在水溶液中自组装成不同尺寸的纳米带、纳米纤维或纳米带的结构异构phdc。基于荧光显微镜结合流式细胞术和共聚焦成像，H9C2细胞对尼罗河红负载的phdc的摄取表现出形态依赖性，其中狭窄的螺旋状扭曲纳米带最有效地进入。所有PHDC释放H2S的速率相似，但细胞内释放H2S的量取决于每个PHDC的内化能力。与这些结果一致，窄扭曲纳米带对h2o2诱导的氧化应激具有最大的保护作用。总的来说，本研究强调了细微的分子水平变化如何影响超分子组装中纳米结构的形成，并最终影响其细胞摄取和生物活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Materials Letters MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.60

自引率

3.50%

发文量

261

期刊介绍： ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.