Pluronic F127 nanoparticles modified with folate and loaded with phycocyanobilin for tumor-targeted photodynamic therapy

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-08-27 DOI:10.1016/j.dyepig.2025.113190

Xuxiang Cai , Yuhui Hu , Siqi Xiao , Yunquan Zheng , Jianmin Yang , Xianai Shi , Chengqun Chen

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引用次数: 0

Abstract

Photodynamic therapy (PDT) has emerged as a promising cancer treatment strategy due to its minimally invasive nature and high specificity for malignant tissue. Our previous work has shown that phycocyanobilin (PCB), a natural food coloring derived from Arthrospira Platensis, has the potential to be a photosensitizer. Compared to most reported photosensitizers, PCB is almost non-toxic and readily available. However, its water insolubility and inadequate targeting delivery to cancer cells have been identified as significant obstacles, which compromise its bioavailability and further clinical application. In the present study, Folate-modified pluronic F127 nanoparticle drug delivery system (FF) was developed, which possessed specific tumor targeting, homogeneity, dispersibility and good biocompatibility. This system has been shown to enhance PCB uptake by 4T1 mouse breast carcinoma cells, increase the intracellular ROS production, induce 4T1 cell apoptosis in a concentration-dependent manner, and significantly improve the anticancer activity of PCB. The IC₅₀ value of Phycocyanobilin-Folate acid-pluronic F127 nanoparticle drug delivery system (PFF) under light irradiation was determined to be 42.75 ± 0.78 μmol/L, which was 26.62 % higher than that of PCB alone. Zn-Phthalocyanine is a typical second-generation photosensitizer. The therapeutic index (TI) value of PFF was found to be 8.09 under light irradiation, which was significantly higher than that of Zn-Phthalocyanine (TI = 1.08). In vivo studies have shown the efficacy of PFF in delivering PCB to tumor sites, resulting in a significant inhibition rate of tumor growth inhibition (96.15 ± 3.70 %). Furthermore, PFF has been demonstrated to exhibit excellent biocompatibility, suggesting its potential for use in clinical treatments.

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叶酸修饰的Pluronic F127纳米颗粒，装载藻蓝胆素用于肿瘤靶向光动力治疗

光动力疗法（PDT）因其微创性和对恶性组织的高特异性而成为一种很有前途的癌症治疗策略。我们之前的工作已经表明，藻蓝胆素（PCB）是一种从platarthrospira Platensis中提取的天然食用色素，具有光敏剂的潜力。与大多数报道的光敏剂相比，多氯联苯几乎无毒且易得。然而，其不溶于水和不适合靶向递送癌细胞已被确定为重大障碍，这影响了其生物利用度和进一步的临床应用。本研究开发了叶酸修饰的pluronic F127纳米颗粒给药系统（FF），该系统具有特异性肿瘤靶向性、均匀性、分散性和良好的生物相容性。该系统已被证明可以增强4T1小鼠乳腺癌细胞对PCB的摄取，增加细胞内ROS的产生，诱导4T1细胞以浓度依赖的方式凋亡，并显著提高PCB的抗癌活性。测定了藻蓝胆素-叶酸酸-pluronic F127纳米颗粒给药体系（PFF）在光照下的IC50值为42.75±0.78 μmol/L，比单独使用PCB的IC50值高26.62%。酞菁锌是典型的第二代光敏剂。光照射下PFF的治疗指数（TI）值为8.09，显著高于锌-酞菁（TI = 1.08）。体内研究表明，PFF可将PCB递送至肿瘤部位，对肿瘤生长抑制率显著（96.15±3.70%）。此外，PFF已被证明具有良好的生物相容性，这表明其在临床治疗中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.