The association between glyphosate use and mosaic loss of chromosome Y in buccal samples among male pesticide applicators in the Agricultural Health Study

IF 9.7 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environment International Pub Date : 2025-09-01 DOI:10.1016/j.envint.2025.109755

Vicky C. Chang , Stella Koutros , Lauren M. Hurwitz , Weiyin Zhou , Gabriella Andreotti , Christine G. Parks , Dale P. Sandler , Nathaniel Rothman , Mitchell J. Machiela , Laura E. Beane Freeman , Jonathan N. Hofmann , Sonja I. Berndt

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Abstract

Glyphosate-based herbicides are the most widely applied pesticides worldwide and have been implicated in the development of certain hematologic malignancies; however, the underlying biological mechanisms are not well-understood. High lifetime use of glyphosate-based herbicides, hereafter referred to as glyphosate, was previously associated with mosaic loss of chromosome Y (mLOY), a biomarker of genomic instability potentially linked to cancer and immune dysregulation, in circulating blood of male farmers from a subcohort of the Agricultural Health Study (AHS). Here, we further investigated the association between glyphosate use and mLOY using buccal-derived DNA among 1,868 male pesticide applicators in an independent AHS study. We detected mLOY using genotyping array intensity data in the male-specific region of the Y chromosome. Information on cumulative lifetime use of glyphosate was derived from questionnaires. Logistic regression analyses were used to estimate odds ratios (ORs) and 95 % confidence intervals (CIs) for the associations of glyphosate use with overall mLOY and mLOY affecting a high cell fraction (≥ median), adjusted for potential confounders. Overall, mLOY was detected in buccal samples of 298 (16 %) pesticide applicators. High intensity-weighted lifetime days of glyphosate use was associated with overall mLOY (OR_{highest vs. lowest quartile of glyphosate use} = 1.54; 95 % CI: 1.01, 2.37), although the exposure–response trend was not statistically significant (P_trend = 0.40). For mLOY affecting a high fraction of cells, positive associations were observed for both high lifetime days (OR_{highest vs. lowest quartile of glyphosate use} = 1.76; 95 % CI: 1.04, 2.99; P_trend = 0.06) and high intensity-weighted lifetime days (OR_{highest vs. lowest quartile of glyphosate use} = 2.02; 95 % CI: 1.12, 3.64; P_trend = 0.16). Together, these findings support previous evidence linking glyphosate and mLOY and help inform our understanding of the biological impact of glyphosate on human health and its potential role in carcinogenesis.

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农业健康研究中男性农药施药者口腔样本中草甘膦使用与Y染色体花叶丢失之间的关系

草甘膦除草剂是世界上应用最广泛的农药，与某些血液恶性肿瘤的发展有关；然而，其潜在的生物学机制尚不清楚。在农业健康研究（AHS）的一个亚队列研究中，长期使用草甘膦除草剂（以下简称草甘膦）与男性农民循环血液中染色体Y （mLOY）的马赛克缺失有关，染色体Y （mLOY）是基因组不稳定的生物标志物，可能与癌症和免疫失调有关。在一项独立的AHS研究中，我们利用口腔DNA进一步调查了1868名男性农药施药者使用草甘膦与mLOY之间的关系。我们利用基因分型阵列强度数据在Y染色体的男性特异性区域检测mLOY。关于终生累积使用草甘膦的信息来自问卷调查。使用Logistic回归分析来估计草甘膦使用与总体mLOY和影响高细胞分数（≥中位数）的mLOY之间的比值比（ORs）和95% %置信区间（CIs），并对潜在混杂因素进行了调整。总体而言，在298名（16% %）施用农药者的口腔样本中检测到mLOY。使用草甘膦的高强度加权寿命天数与总体mLOY相关（草甘膦使用的最高四分位数vs最低四分位数 = 1.54;95 % CI: 1.01, 2.37），尽管暴露-反应趋势无统计学意义（p趋势 = 0.40）。mLOY影响高分数的细胞,观察高一生天积极联系(ORhighest与最低四分位数的草甘膦使用 = 1.76;95 % CI: 1.04, 2.99; Ptrend = 0.06)和高intensity-weighted一生天(ORhighest与最低四分位数的草甘膦使用 = 2.02;95 % CI: 1.12, 3.64; Ptrend = 0.16)。总之，这些发现支持了先前将草甘膦与mLOY联系起来的证据，并有助于我们了解草甘膦对人类健康的生物学影响及其在致癌中的潜在作用。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.