Marker Assisted Introgression and Evaluation of Retinol Equivalent Carotenoid in the Elite Cultivars Background from Traditional Landrace ‘Kavuni’

IF 1.1 Q3 AGRONOMY

Agricultural Research Pub Date : 2024-11-06 DOI:10.1007/s40003-024-00789-5

Suvarna Rani Chimmili, G. Subashini, M. Raveendran, Anandakumar C. R., Jyothi Badri, Divya Balakrishnan, S. Robin

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Abstract

Bioactive compound ‘lutein’, an α-carotenoid is responsible for ocular health and preventing major cardiovascular diseases in human beings and it cannot be synthesized de novo. ‘Lutein content is found only in traces among the popular cultivars of rice. ‘Kavuni’, an Indian traditional landrace, rich in lutein content (238 μg/100 g) is used in the present study to transfer lutein into two popular cultivars ‘ASD 16’ and ‘Swarna Sub1’ using marker assisted backcross breeding strategy. Phenotyping for lutein quantification was done using HPLC (High performance Liquid Chromatography) and further antioxidant analysis was carried out by DPPH (1, 1-diphenyl-2-picryl-hydrazyl) based scavenging assay. Across the crosses, eight backcross progeny were found to have higher lutein content than respective recurrent parents with an average lutein content of 96.5 μg/100 g and background recovery of 64.53%. One progeny (S33) from Cross II recorded highest lutein content of 322 μg/100 g, much higher than the donor parent indicating transgressive segregation. Significant positive correlation was observed between lutein content and antioxidants among the backcross progeny.

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传统地方品种“卡乌尼”优良品种背景下视黄醇当量类胡萝卜素的标记辅助导入及评价

生物活性化合物“叶黄素”是一种类α-胡萝卜素，对人类的眼部健康和预防主要心血管疾病起着重要作用，不能从头合成。叶黄素的含量在普通水稻品种中只发现了微量。本研究采用标记辅助回交育种策略，将叶黄素含量高（238 μg/100 g）的印度传统地方品种“Kavuni”转入两个热门品种“ASD 16”和“Swarna Sub1”。采用高效液相色谱法（HPLC）对叶黄素进行表型分析，并采用基于DPPH（1,1 -二苯基-2-苦基-肼基）的清除法进行进一步的抗氧化分析。8个回交后代的叶黄素含量均高于各自的回交亲本，平均叶黄素含量为96.5 μg/100 g，背景回收率为64.53%。杂交II的一个子代（S33）叶黄素含量最高，为322 μg/100 g，远高于供体亲本，表明存在海侵分离。回交后代叶黄素含量与抗氧化剂含量呈显著正相关。

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来源期刊

Agricultural Research AGRONOMY-

CiteScore

3.80

自引率

0.00%

发文量

期刊介绍： The main objective of this initiative is to promote agricultural research and development. The journal will publish high quality original research papers and critical reviews on emerging fields and concepts for providing future directions. The publications will include both applied and basic research covering the following disciplines of agricultural sciences: Genetic resources, genetics and breeding, biotechnology, physiology, biochemistry, management of biotic and abiotic stresses, and nutrition of field crops, horticultural crops, livestock and fishes; agricultural meteorology, environmental sciences, forestry and agro forestry, agronomy, soils and soil management, microbiology, water management, agricultural engineering and technology, agricultural policy, agricultural economics, food nutrition, agricultural statistics, and extension research; impact of climate change and the emerging technologies on agriculture, and the role of agricultural research and innovation for development.