Catalytic performance of lipase-inorganic hybrid nanoflower in the presence of some oils/fat/waste oils and detergents/surfactants

IF 2.5 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2025-06-20 DOI:10.1007/s11696-025-04168-4

Kezban Yildiz-Dalginli

{"title":"Catalytic performance of lipase-inorganic hybrid nanoflower in the presence of some oils/fat/waste oils and detergents/surfactants","authors":"Kezban Yildiz-Dalginli","doi":"10.1007/s11696-025-04168-4","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, the conventional hybrid nanoflower synthesis method was used for lipase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, their activity was investigated in some detergents/surfactants and oils/fats/waste oils to evaluate the activity, stability and compatibility of the biocatalyst. The optimal morphology for L@ihNFs was determined to be 0.05 mg/mL. To obtain the optimum activity, conditions were explored pH values ranging from 6 to 12 and temperatures between 30 and 80 °C. Both the free form and L@ihNF exhibited higher activity in alkaline conditions, with a significant decrease in activity observed at pH 11 and 12. L@ihNF showed superior activity and remarkable compatibility compared to the free enzyme in the presence of various commercial liquid/powder detergents and surfactants. Moreover, it displayed over a twofold increase in activity in some oils/fat/waste oils. The reusability of the synthesized L@ihNF was examined in 10 cycles, and it was observed that it still showed 58% activity at the end of the 8th cycle, which is a good indicator of reusability. L@ihNFs exhibited good storage stability, retaining approximately 50% activity after 20 days compared to the free enzyme.</p></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":"79 9","pages":"5997 - 6010"},"PeriodicalIF":2.5000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-025-04168-4","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, the conventional hybrid nanoflower synthesis method was used for lipase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, their activity was investigated in some detergents/surfactants and oils/fats/waste oils to evaluate the activity, stability and compatibility of the biocatalyst. The optimal morphology for L@ihNFs was determined to be 0.05 mg/mL. To obtain the optimum activity, conditions were explored pH values ranging from 6 to 12 and temperatures between 30 and 80 °C. Both the free form and L@ihNF exhibited higher activity in alkaline conditions, with a significant decrease in activity observed at pH 11 and 12. L@ihNF showed superior activity and remarkable compatibility compared to the free enzyme in the presence of various commercial liquid/powder detergents and surfactants. Moreover, it displayed over a twofold increase in activity in some oils/fat/waste oils. The reusability of the synthesized L@ihNF was examined in 10 cycles, and it was observed that it still showed 58% activity at the end of the 8th cycle, which is a good indicator of reusability. L@ihNFs exhibited good storage stability, retaining approximately 50% activity after 20 days compared to the free enzyme.

查看原文本刊更多论文

脂肪酶-无机杂化纳米花在油/脂肪/废油和洗涤剂/表面活性剂存在下的催化性能

本研究采用常规的杂化纳米花合成方法，制备了脂肪酶-无机杂化纳米花。在对纳米花进行全面的pH和温度扫描后，研究了它们在一些洗涤剂/表面活性剂和油/脂肪/废油中的活性，以评估生物催化剂的活性、稳定性和相容性。确定L@ihNFs的最佳形态为0.05 mg/mL。为了获得最佳活性，研究了pH值为6 ~ 12，温度为30 ~ 80℃的条件。游离态和L@ihNF在碱性条件下均表现出较高的活性，pH值为11和12时活性显著降低。L@ihNF与游离酶相比，在各种商业液体/粉末洗涤剂和表面活性剂存在下表现出优越的活性和显著的相容性。此外，在一些油/脂肪/废油中，它的活性增加了两倍以上。对合成的L@ihNF进行了10次循环的可重用性测试，观察到在第8次循环结束时，其活性仍为58%，这是一个很好的可重用性指标。L@ihNFs表现出良好的储存稳定性，与游离酶相比，20天后保持约50%的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.