Diversity of Root Endophytic Fungi from Some Medicinal Plants of Papaveraceae in Iran

IF 1.1 Q3 AGRONOMY
Yasaman Tajik Gharibi, Kamran Rahnama, Amir Zolfaghary, Khodayar Hemmati, Afsaneh Graan
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引用次数: 0

Abstract

Papaveraceae encompasses some of the most significant and widely utilized plants within traditional medicine, pharmaceuticals, and food industries. This study aims to delineate the diversity of root endophytic fungi associated with Glaucium fimbrilligerum, G. contortuplicatum, Chelidonium majus, Papaver macrostomum, P. chelidoniifolium, P. pavoninum, and P. rhoeas from Iran. Molecular identification involved amplification and sequencing of LSU (partial large subunit nrDNA), ITS (internal transcribed spacer), TEF-1α (translation elongation factor), and TUB (β-tubulin) genomic regions. A total of 32 isolates of endophytic fungi were identified. Approximately 96.87% of these fungi belonged to Ascomycota, with only Irpex laceratus isolated and identified from G. fimbrilligerum as Basidiomycota. Fusarium was the predominant genus, representing 34.37% of endophytic fungi, followed by Sarocladium, Alternaria, and Cladosporium at 18.75%, 9.375%, and 6.25%, respectively. F. proliferatum was found to colonize 71.42% of plants, including P. macrostomum, P. chelidoniifolium, P. pavoninum, P. rhoeas, and C. majus. The fungus isolate S. strictum was recovered from 42.85% of various plant species, including G. fimbrilligerum, G. contortuplicatum, and P. chelidoniifolium. Moreover, this investigation represents the inaugural documentation of endophytic behavior exhibited by the fungi A. japonica and S. implicatum within plant species, as no previous literature has reported such occurrences. Consequently, this study unveils novel insights into the population of endophytic fungi associated with seven medicinal plants belonging to the Papaveraceae family. These findings not only enrich our understanding of fungal biodiversity but also hold implications for species conservation efforts and advancements in elucidating the intricate dynamics of plant-microbiome interactions within their native ecosystems.

伊朗部分罂粟科药用植物根系内生真菌多样性研究
罂粟科包括一些在传统医药、制药和食品工业中最重要和广泛应用的植物。本研究旨在研究伊朗产的青光带(Glaucium fibribriigerum)、弯纹葡萄(g.c orortuplicatum)、大Chelidonium majus、Papaver macrostomum、P. chelidoniiolium、P. pavoninum和P. rhoeas根系内生真菌的多样性。分子鉴定包括LSU(部分大亚基nrDNA)、ITS(内部转录间隔物)、TEF-1α(翻译延伸因子)和TUB (β-微管蛋白)基因组区域的扩增和测序。共鉴定出内生真菌32株。这些真菌中约96.87%属于子囊菌门,仅从G. fibrbriigerum中分离鉴定为担子菌门的Irpex laceratus。镰刀菌属为优势属,占内生真菌总数的34.37%,其次为细枝菌属(18.75%)、交替菌属(9.375%)和枝孢菌属(6.25%)。结果表明,71.42%的植物均有增殖力粉虱的定殖,包括大气孔粉虱、chelidonifolium粉虱、pavoninum粉虱、rhoeas粉虱和majus粉虱。从各种植物中分离出了42.85%的狭窄葡萄球菌(S. strictum),包括G. fibribriigerum、G. contortuplicatum和P. chelidonifolium。此外,该研究首次记录了植物物种中A. japonica和S. implicatum真菌的内生行为,此前没有文献报道过此类事件。因此,本研究揭示了与Papaveraceae家族的七种药用植物相关的内生真菌种群的新见解。这些发现不仅丰富了我们对真菌生物多样性的理解,而且对物种保护工作和阐明原生生态系统中植物-微生物相互作用的复杂动态具有重要意义。
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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
24
期刊介绍: The main objective of this initiative is to promote agricultural research and development. The journal will publish high quality original research papers and critical reviews on emerging fields and concepts for providing future directions. The publications will include both applied and basic research covering the following disciplines of agricultural sciences: Genetic resources, genetics and breeding, biotechnology, physiology, biochemistry, management of biotic and abiotic stresses, and nutrition of field crops, horticultural crops, livestock and fishes; agricultural meteorology, environmental sciences, forestry and agro forestry, agronomy, soils and soil management, microbiology, water management, agricultural engineering and technology, agricultural policy, agricultural economics, food nutrition, agricultural statistics, and extension research; impact of climate change and the emerging technologies on agriculture, and the role of agricultural research and innovation for development.
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