Tusconia apicata gen. et sp. nov.: An innovative biomass source with antioxidant and anti-inflammatory potential from thermal spring Bagni San Filippo

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-08-18 DOI:10.1016/j.algal.2025.104267

Teresa De Rosa , Guilherme Scotta Hentschke , Graciliana Lopes , Flavio Oliveira , Germana Esposito , Roberta Teta , Vitor Vasconcelos , Valeria Costantino

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Abstract

Thermal springs represent distinctive ecosystems that harbor various cyanobacterial communities adapted to unique physicochemical conditions. This paper describes Tusconia apicata gen. et sp. nov. TBCL 2401, a novel cyanobacterium isolated from the Bagni San Filippo hot springs in Tuscany, Italy. Our interdisciplinary approach, which merges phylogenetic analyses with chemical analyses and antioxidant tests, unveiled the potential of this biomass resource.

Phylogenetic analysis based on the 16S rRNA gene positioned T. apicata within the Oculatellales order, showing a distinct relationship with Toxifilum, Sodaleptolyngbya, and Eurychoronema. This classification was further supported by 16S23S ITS secondary structure analysis and morphological and pigment profiling.

High-Performance Liquid Chromatography with Photodiode Array detection (HPLC-PDA) analysis of the acetonic extract revealed a high concentration of carotenoids, with β-carotene (7.40 μg mg⁻¹) as the predominant compound. This extract exhibited significant anti-inflammatory potential by reducing nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Conversely, spectrophotometric quantification of the aqueous extract confirmed a high phycocyanin content (156.55 μg mg⁻¹), which contributed to its antioxidant activity, as demonstrated by its ability to scavenge nitric oxide (^•NO) (IC₂₅ = 113.24 μg mL⁻¹) and superoxide anion (O₂^•-) (IC₂₅ = 75.31 μg mL⁻¹) radicals. These results suggest that T. apicata’ metabolites may play a role in modulating inflammatory and oxidative stress responses.

Moreover, the absence of cyanotoxin biosynthetic genes underscores the potential safety of T. apicata gen. et sp. nov. TBCL 2401 for biotechnological applications. The discovery of this new genus enriches our knowledge of cyanobacterial diversity in thermal environments and highlights their relevance as sources of bioactive compounds with promising pharmaceutical and industrial applications.

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来自巴格尼圣菲利波温泉的具有抗氧化和抗炎潜力的创新生物质资源

温泉代表独特的生态系统，港湾各种蓝藻群落适应独特的物理化学条件。本文报道了一种从意大利托斯卡纳的Bagni San Filippo温泉中分离出来的新型蓝藻Tusconia apicata gen. et sp. nov. TBCL 2401。我们的跨学科方法，将系统发育分析与化学分析和抗氧化测试相结合，揭示了这种生物质资源的潜力。基于16S rRNA基因的系统发育分析将T. apicata定位为Oculatellales目，与Toxifilum、sodalepolyynbya和Eurychoronema有明显的亲缘关系。16S23S ITS二级结构分析、形态学和色素谱分析进一步支持了这一分类。高效液相色谱-光电二极管阵列检测（HPLC-PDA）分析显示，丙酮提取物中含有高浓度的类胡萝卜素，主要化合物为β-胡萝卜素（7.40 μg mg−1）。该提取物通过减少脂多糖（LPS）刺激的RAW 264.7巨噬细胞中一氧化氮的产生，显示出显著的抗炎潜力。相反，水提取物的分光光度定量证实了高藻蓝蛋白含量（156.55 μg mg - 1），这有助于其抗氧化活性，因为它能够清除一氧化氮（•NO）（IC₂₅= 113.24 μg mL - 1）和超氧阴离子（O₂•-）（IC₂₅= 75.31 μg mL - 1）自由基。上述结果提示，凤头藤代谢物可能在调节炎症和氧化应激反应中发挥作用。此外，蓝藻毒素生物合成基因的缺失强调了T. apicata gen. et sp. nov. TBCL 2401在生物技术应用方面的潜在安全性。这个新属的发现丰富了我们对热环境中蓝藻多样性的认识，并突出了它们作为有前途的制药和工业应用的生物活性化合物来源的相关性。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment