Effects of dietary distiller's grains on growth and gut microbiota of the sea cucumber Apostichopus japonicus

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-08-30 DOI:10.1016/j.aqrep.2025.103057

Weiyan Li , Haoran Xiao , Shufeng Li , Jiayi Zhang , Yajie Deng , Ziyu Liu , Lingshu Han , Junxiao Sun , Yaqing Chang , Jun Ding

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Abstract

This study evaluated the impacts of dietary distiller's grains (DG) on the growth, nutritional quality, and intestinal microbiota of Apostichopus japonicus. The healthy A. japonicus (21.51 ± 0.64 g initial weight) were allocated to four groups (three replicates each): a basal diet (T0) and three DG-supplemented diets: 10 % powder (T1), 20 % powder (T2), and 5 % liquid (T3), for 60 days trial. Growth parameters, nutrient profiles, and microbial communities were analyzed post-experiment. The group T3 exhibited significantly higher final body weight, weight gain rate, and specific growth rate versus controls (P < 0.05), outperforming T1 and T2. Nutritional analysis revealed elevated crude protein in group T3 (P < 0.05). Electron microscopy demonstrated denser collagen fiber bundles in group T3 compared to T1 and T2. Gut microbiota sequencing indicated distinct structural shifts in group T3, with increased Verrucomicrobia abundance (P < 0.05) and altered community similarity relative to controls (P < 0.05). In comparison, T2 enhanced microbial alpha diversity (P < 0.05), powdered DG (T1 and T2) impaired collagen synthesis and body wall quality despite promoting growth. In contrast, 5 % liquid DG supplementation (T3) optimized growth performance through protein enrichment and beneficial microbial modulation, particularly Verrucomicrobia proliferation. These findings demonstrate that liquid DG enhances A. japonicus aquaculture sustainability by improving growth metrics and nutritional value while modulating the gut microbial structure.

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日粮中添加酒糟对刺参生长和肠道菌群的影响

本研究评价了日粮中添加酒糟对日本刺参生长、营养品质和肠道菌群的影响。选取初重21.51 ± 0.64 g的健康日本鳗鲡，分为4组（每组3个重复），分别饲喂基础饲粮（T0）和饲粮中添加10 %粉剂（T1）、20 %粉剂（T2）和5 %液体（T3），试验期60 d。实验结束后，对生长参数、营养成分和微生物群落进行分析。T3组的末重、增重率和特定生长率均显著高于对照组（P <； 0.05），优于T1和T2组。营养分析显示T3组粗蛋白质含量升高（P <； 0.05）。电镜显示T3组胶原纤维束较T1和T2组致密。肠道菌群测序显示T3组有明显的结构变化，Verrucomicrobia丰度增加（P <； 0.05），群落相似性相对于对照组改变（P <； 0.05）。相比之下，T2增强了微生物α多样性（P <； 0.05），粉末DG （T1和T2）虽然促进了生长，但却损害了胶原合成和体壁质量。相比之下，添加5 %液体DG （T3）通过蛋白质富集和有益微生物调节，特别是Verrucomicrobia增殖，优化了生长性能。综上所示，液态DG可通过调节肠道微生物结构，改善刺参的生长指标和营养价值，从而提高刺参养殖的可持续性。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.