Ultrasound-assisted deep eutectic solvent extraction of polysaccharides from Cercis chinensis bark: Optimization, kinetics and antioxidant activities

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-08-26 DOI:10.1016/j.ultsonch.2025.107535

Penghua Shu , Na Wang , Yu Meng , Shuxian Fan , Simin Liu , Xinfeng Fan , Di Hu , Hao Yin , Huiying Wang , Xin Wang , Mengjiao Wu , Feng Yu , Xialan Wei , Lin Zhang , Jihong Huang

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Abstract

This study pioneers a green, efficient ultrasound-assisted extraction (UAE) technique using a deep eutectic solvent (DES) to isolate polysaccharides from Cercis chinensis bark, overcoming limitations of conventional methods. Through systematic screening of 15 DES systems, DES-11 (L-proline:ethylene glycol, 1:2 M ratio) was identified as optimal, achieving a polysaccharide yield of 64.7 mg/g—3.7-fold higher than water extraction. Extraction conditions were optimized via response surface methodology (RSM) to 39 % water content, 45 °C, 28 min, solid–liquid ratio 1:40 g/mL, and 240 W ultrasonic power. SEM analysis confirmed that DES-11 disrupts plant cell walls, enhancing mass transfer. Kinetic studies revealed the process follows pseudo-first-order kinetics (R² > 0.90) and is primarily controlled by intraparticle diffusion, with an apparent activation energy of 10.28 kJ·mol⁻¹. The DES-11 extracted polysaccharides exhibited moderate antioxidant activity, with IC₅₀ values of 0.54 mg/mL (DPPH), 0.24 mg/mL (ABTS) and 0.62 mg/mL (OH), suggesting potential as natural food preservatives. DES recyclability was validated over five cycles, retaining > 78 % efficiency. This work establishes DES-UAE as a sustainable strategy for high-yield polysaccharide extraction, with mechanistic insights into kinetics and diffusion control.

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超声辅助深共晶溶剂提取紫荆树树皮多糖：优化、动力学及抗氧化活性

本研究开创了一种绿色、高效的超声辅助提取（UAE）技术，利用深度共晶溶剂（DES）从紫荆树树皮中分离多糖，克服了传统方法的局限性。通过对15个DES体系的系统筛选，确定DES-11 （l -脯氨酸：乙二醇，1:2 M比）为最佳体系，多糖得率为64.7 mg/g，比水提法高3.7倍。采用响应面法（RSM）优化提取条件：水含量39%，温度45℃，时间28 min，料液比1:40 g/mL，超声功率240 W。扫描电镜分析证实，DES-11破坏植物细胞壁，增强传质。动力学研究表明，该过程符合准一级动力学（R2 > 0.90），主要受颗粒内扩散控制，表观活化能为10.28 kJ·mol−1。DES-11提取的多糖具有中等的抗氧化活性，IC50值分别为0.54 mg/mL （DPPH）、0.24 mg/mL （ABTS）和0.62 mg/mL (OH)，具有作为天然食品防腐剂的潜力。DES的可回收性在5个循环中得到验证，效率保持在78%。这项工作建立了DES-UAE作为高产量多糖提取的可持续策略，具有动力学和扩散控制的机理见解。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.