Ultrasonic engineering of bovine serum albumin nanoparticles for high internal phase Pickering emulsions: Interfacial behavior, microstructural evolution and stabilization enhancement

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-08-28 DOI:10.1016/j.ultsonch.2025.107543

Liyuan Ma , Jie Li , Yixiang Liu , Jie Zheng

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Abstract

This study investigated the influence of ultrasonic treatment on the physicochemical properties of bovine serum albumin (BSA) and its applicability in stabilizing high internal phase Pickering emulsions (HIPPEs). Under optimized sonication conditions (250 W, 12 min), stable ultrasonically modified BSA (UBSA) particles were generated, exhibiting a small particle size (41.39 nm), a polydispersity index < 0.17, a higher absolute zeta potential (> 20 mV), and favorable wettability (three-phase contact angle: 78.71°), accompanied by reduced surface hydrophobicity. Intrinsic fluorescence spectra and circular dichroism (CD) analysis confirmed that ultrasonication altered the secondary structure of BSA, leading to the exposure of hydrophobic groups. Dynamic interfacial tension and adsorption kinetics analyses revealed that UBSA particles exhibited lower interfacial tension and significantly higher diffusion coefficient (K_diff) and penetration coefficient (K_p) than native BSA, indicating enhanced diffusion and adsorption capabilities of UBSA at the oil–water interface. Rheological analyses demonstrated that UBSA-stabilized HIPPEs possessed higher viscosity and larger storage (G′) and loss (G″) moduli. Optical and confocal laser scanning microscopy confirmed the successful formation of HIPPEs at UBSA concentrations ≥ 1.0 % (w/v). UBSA-stabilized HIPPEs displayed a reduced droplet size (10.59 µm) and a more densely packed droplet structure, which conferred enhanced resistance against droplet coalescence compared to emulsions stabilized by native BSA. Moreover, stability assessments indicated that the centrifugal, freeze–thaw and storage stability of the prepared HIPPEs were significantly improved. Importantly, UBSA-based HIPPEs serving as a delivery vehicle also effectively enhanced the thermal processing stability of β-carotene. The findings demonstrate the potential of ultrasound-modified BSA nanoparticles as effective stabilizers for HIPPEs, providing valuable insights for the development of healthy and safe food-grade emulsion systems.

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用于高内相皮克林乳剂的牛血清白蛋白纳米粒子的超声工程：界面行为、微观结构演变和稳定性增强

研究了超声处理对牛血清白蛋白（BSA）理化性质的影响及其在稳定高内相皮克林乳剂（hipes）中的适用性。在优化的超声处理条件下（250 W, 12 min），可制得稳定的超声修饰BSA （UBSA）颗粒，其粒径小（41.39 nm），多分散性指数<； 0.17，绝对zeta电位较高（> 20 mV），润湿性好（三相接触角78.71°），表面疏水性降低。本征荧光光谱和圆二色性（CD）分析证实，超声改变了牛血清白蛋白的二级结构，导致疏水性基团暴露。动态界面张力和吸附动力学分析表明，与天然BSA相比，UBSA颗粒的界面张力更低，扩散系数（Kdiff）和渗透系数（Kp）显著提高，表明UBSA在油水界面的扩散和吸附能力增强。流变学分析表明，ubsa稳定的hipes具有更高的粘度和更大的储存（G '）和损失（G″）模量。光学和共聚焦激光扫描显微镜证实，当UBSA浓度≥1.0% （w/v）时，hipes成功形成。与天然BSA稳定的乳剂相比，ubsa稳定的hipes显示出更小的液滴尺寸（10.59µm）和更致密的液滴结构，从而增强了抗液滴聚结的能力。稳定性评价表明，制备的hipes的离心稳定性、冻融稳定性和贮藏稳定性均有显著提高。重要的是，基于ubsa的hipes作为递送载体也有效地增强了β-胡萝卜素的热处理稳定性。研究结果表明，超声修饰的牛血清白蛋白纳米颗粒具有作为高效hipes稳定剂的潜力，为开发健康安全的食品级乳液体系提供了有价值的见解。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.