OsMATE7-mediated flavonol accumulation regulates pollen tube growth in rice

IF 5.7 1区生物学 Q1 PLANT SCIENCES

The Plant Journal Pub Date : 2025-08-28 DOI:10.1111/tpj.70449

Shijun Fan, Kailai Huang, Yan Yang, Yi Xi, Huan Ye, Lei Xu, Weilan Chen, Peng Gao, Ting Li, Bin Tu, Hua Yuan, Bingtian Ma, Yuping Wang, Zhaohui Zhong, Jiawei Xiong, Liangzhu Kang, Shiwen Tang, Xuewei Chen, Chengbin Xiang, Shigui Li, Peng Qin

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Abstract

Flavonols have been implicated in male sterility and pollen tube growth for over three decades; however, the molecular mechanisms mediating their accumulation in pollen grains remain poorly understood. In this study, a multidrug and toxic compound extrusion (MATE) transporter, OsMATE7, was identified as a key regulator of flavonol accumulation in mature pollen grains, thereby promoting pollen tube growth in rice (Oryza sativa). Mutation of OsMATE7 resulted in a significant reduction in seed setting rates. Reciprocal crosses between OsMATE7 knockout (KO) and wild-type plants, along with both in vivo and in vitro germination assays, demonstrated that the mutant exhibited male sterility due to defective pollen tube growth. OsMATE7 was found to be specifically expressed in pollen and localized to the endoplasmic reticulum (ER). Diphenylboric acid 2-aminoethylester (DPBA) staining assay revealed a significant reduction in flavonol accumulation in mature pollen grains of OsMATE7. Functional analysis of flavonol transport and flavonoid content confirmed that OsMATE7 serves as a potential flavonol transporter, facilitating the accumulation of flavonols in mature pollen grains. Further investigation revealed that flavonol deficiency in mutant pollen grains inhibited α-amylase activity and blocked starch hydrolysis, ultimately impairing pollen tube growth. These findings elucidate a novel mechanism by which OsMATE7 regulates flavonol distribution to control pollen tube growth, providing new insights into the role of MATE proteins in seed setting and crop breeding.

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osmate7介导的黄酮醇积累调控水稻花粉管生长

三十多年来，黄酮醇一直与雄性不育和花粉管生长有关；然而，介导它们在花粉粒中积累的分子机制仍然知之甚少。本研究发现，一种多药毒性复合物挤压（MATE）转运蛋白OsMATE7是水稻成熟花粉粒中黄酮醇积累的关键调控因子，从而促进花粉管生长。OsMATE7突变导致结实率显著降低。OsMATE7基因敲除（KO）与野生型植物的互交，以及体内和体外萌发试验表明，由于花粉管生长缺陷，该突变体表现出雄性不育性。发现OsMATE7在花粉中特异表达，定位于内质网（ER）。二苯硼酸2-氨基乙基酯（DPBA）染色显示，OsMATE7成熟花粉粒中黄酮醇积累显著减少。对黄酮醇转运和类黄酮含量的功能分析证实，OsMATE7可能是一个潜在的黄酮醇转运蛋白，促进黄酮醇在成熟花粉粒中的积累。进一步研究发现，突变体花粉缺乏黄酮醇抑制α-淀粉酶活性，阻断淀粉水解，最终损害花粉管生长。这些发现阐明了OsMATE7调控黄酮醇分布控制花粉管生长的新机制，为MATE蛋白在种子结实和作物育种中的作用提供了新的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.