Biodepolymerisations of intracellular and extracellular polyhydroxyalkanoates: Methodological approaches and current update on biotechnological applications

IF 7.4 2区化学 Q1 POLYMER SCIENCE

Polymer Degradation and Stability Pub Date : 2025-08-25 DOI:10.1016/j.polymdegradstab.2025.111626

Nur Hanani Arif Shah , Siti Nor Syairah Anis , Amirul Al-Ashraf Abdullah , Mohamad Suffian Mohamad Annuar , Khanom Simarani , Ida Idayu Muhamad , Sevakumaran Vigneswari

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引用次数: 0

Abstract

Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesised as intracellular carbon and energy storage compounds, with significant potential as sustainable and biodegradable alternatives to petroleum-based plastics. Their degradation, or depolymerisation, occurs through both intracellular and extracellular mechanisms, each offering unique methodological challenges and opportunities. This review provides a comprehensive overview of current methodological approaches for studying PHA depolymerisation via enzymatic hydrolysis, encompassing in vivo and in vitro intracellular depolymerisation, as well as environmental, in vivo, and in vitro extracellular depolymerisation processes. Furthermore, recent advances in the biotechnological applications of PHA depolymerisation products, especially enantiomerically pure (R)-3-hydroxyalkanoates, are discussed. These monomers are increasingly exploited in the production of versatile chiral building blocks with diverse applications, ranging from pharmaceuticals and food additives to biodegradable plastics and plant protection. This review aims to consolidate the current knowledge base and highlight emerging methodological approaches in studying PHA depolymerisation and applications of its depolymerised products.

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细胞内和细胞外聚羟基烷酸酯的生物解聚：方法方法和生物技术应用的最新进展

聚羟基烷酸酯（PHAs）是一种微生物合成的聚酯，作为细胞内碳和能量储存化合物，具有作为石油基塑料的可持续和可生物降解替代品的巨大潜力。它们的降解或解聚合通过细胞内和细胞外机制发生，每种机制都提供了独特的方法挑战和机遇。本文综述了目前通过酶解研究PHA解聚合的方法，包括体内和体外细胞内解聚合，以及环境、体内和体外细胞外解聚合过程。此外，还讨论了PHA解聚产物的生物技术应用的最新进展，特别是对构象纯(R)-3-羟基烷酸酯。这些单体越来越多地用于生产具有不同应用的多功能手性构建块，从药品和食品添加剂到可生物降解塑料和植物保护。这篇综述旨在巩固现有的知识基础，并突出了研究PHA解聚及其解聚产物应用的新兴方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Polymer Degradation and Stability 化学-高分子科学

CiteScore

10.10

自引率

10.20%

发文量

325

审稿时长

23 days

期刊介绍： Polymer Degradation and Stability deals with the degradation reactions and their control which are a major preoccupation of practitioners of the many and diverse aspects of modern polymer technology. Deteriorative reactions occur during processing, when polymers are subjected to heat, oxygen and mechanical stress, and during the useful life of the materials when oxygen and sunlight are the most important degradative agencies. In more specialised applications, degradation may be induced by high energy radiation, ozone, atmospheric pollutants, mechanical stress, biological action, hydrolysis and many other influences. The mechanisms of these reactions and stabilisation processes must be understood if the technology and application of polymers are to continue to advance. The reporting of investigations of this kind is therefore a major function of this journal. However there are also new developments in polymer technology in which degradation processes find positive applications. For example, photodegradable plastics are now available, the recycling of polymeric products will become increasingly important, degradation and combustion studies are involved in the definition of the fire hazards which are associated with polymeric materials and the microelectronics industry is vitally dependent upon polymer degradation in the manufacture of its circuitry. Polymer properties may also be improved by processes like curing and grafting, the chemistry of which can be closely related to that which causes physical deterioration in other circumstances.