Atrial natriuretic peptide-enabled ECM-1 recognition: a new paradigm for Ce3+/Ce4+-amplified breast cancer biosensing

IF 21.8 2区材料科学 Q1 MATERIALS SCIENCE, COMPOSITES

Advanced Composites and Hybrid Materials Pub Date : 2025-08-28 DOI:10.1007/s42114-025-01412-z

Sathish Panneer Selvam, Sungbo Cho

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引用次数: 0

Abstract

Defect-induced cerium oxide (CeO₂) catalysts are highly esteemed in sensing and energy conversion applications. Their catalytic properties are tied to the oxygen vacancies, reflecting efficient redox behavior and stronger adsorption. We designed the calcined ellagic acid (EA) integrated CeO₂ catalyst with an improved number of active sites that favors the remarkable electrochemical response. In addition, the catalyst supports the immobilization of atrial natriuretic peptide (ANP) molecules after being treated with O₂ plasma by inducing the carboxylate formation. The novel approach of combining defect-engineered CeO₂ and ANP contributes to the high sensitivity by providing a favorable environment for extracellular matrix (ECM)-1 binding and efficient electron transfer for signal generation. We contemplated the binding of ECM-1 and ANP with small-angle X-ray scattering profile-mediated molecular docking and studied the dynamic properties. The adsorption energy of − 5.8 eV reveals the stronger binding of the carboxylate group on the CeO₂ surface. Nudged elastic band (NEB) calculations demonstrated that the calcined EA@CeO₂ required only 1.29 eV for breaking the O–H bonding. Furthermore, a 1.67 eV of energy barrier was observed during the O vacancy formation. A lower diffusion rate (1.64 × 10⁻⁴ ps⁻¹) and higher intra-radial distribution function of COOH on CeO₂ established systems with high stability and compactness. The sensor system can detect as low as 0.28 ng mL⁻¹ of ECM-1 with high anti-interference properties and exhibits better performance than conventional antibody-based testing.

Graphical abstract

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心房利钠肽激活的ECM-1识别：Ce3+/Ce4+扩增乳腺癌生物传感的新范式

缺陷诱导氧化铈（CeO2）催化剂在传感和能量转换应用中受到高度重视。它们的催化性能与氧空位有关，反映了有效的氧化还原行为和更强的吸附。我们设计了煅烧鞣花酸（EA）集成CeO2催化剂，提高了活性位点的数量，有利于显著的电化学响应。此外，该催化剂通过诱导羧酸形成，在O2等离子体处理后支持心房钠肽（ANP）分子的固定化。这种结合缺陷工程CeO2和ANP的新方法为细胞外基质(ECM)-1结合提供了有利的环境，并为信号产生提供了有效的电子转移，从而提高了灵敏度。我们设想用x射线小角度散射谱介导的分子对接方法将ECM-1与ANP结合，并研究其动力学性质。- 5.8 eV的吸附能表明羧酸基在CeO2表面的结合更强。微推弹性带（NEB）计算表明，煅烧的EA@CeO2只需要1.29 eV就能破坏O-H键。此外，在O空位形成过程中观察到1.67 eV的能垒。COOH在CeO2上较低的扩散速率（1.64 × 10−4 ps−1）和较高的径向内分布函数使体系具有较高的稳定性和致密性。该传感器系统可检测低至0.28 ng mL−1的ECM-1，具有较高的抗干扰性能，比传统的基于抗体的检测性能更好。图形抽象

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来源期刊

Advanced Composites and Hybrid Materials Multiple-

CiteScore

26.00

自引率

21.40%

发文量

185

期刊介绍： Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.