Ultrasound-intensified wet-heating induced Maillard reaction between hemp cake protein and maltodextrin: Structural characterisation, techno-functional and antioxidant properties
Marzieh Sadeghi , Mohammad Mousavi , Mohammad Saeid Yarmand , Seyed Alireza Salami , Zahra Sarlak , Ehsan Parandi
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引用次数: 0
Abstract
In this research, hemp protein isolate (HPI) was obtained from hemp cake using isoelectric precipitation and conjugated with maltodextrin through the Maillard reaction using wet-heating alone and combined with ultrasound to enhance its structural, functional properties (including solubility, emulsifying, foaming properties and water/oil holding capcity), and antioxidant properties. Covalent bonds between HPI and maltodextrin were confirmed using methods like SDS-PAGE, FTIR, XRD, fluorescence spectroscopy, secondary structure analysis, amino acid profile, and glycation degree (DG) measurement. Ultrasound significantly accelerated glycation, reaching a DG of 25.06 % in just 60 min, while reducing melanoidin formation, whereas wet-heating required 24 h to achieve a similar level of DG. Structural analysis revealed that wet-heating resulted in greater structural modifications due to its longer reaction time. These changes included a decrease in α-helix and β-sheet structures, higher surface hydrophobicity and zeta potential, and more noticeable microstructural changes. Techno-functional assessments revealed that wet-heating conjugates exhibited superior solubility (up to 95.77 %), emulsifying (emulsion stability index up to 48.67 %), and foaming (foaming capacity up to 107.14 %) properties, and antioxidant properties (71.95 % of ABTS), compared to ultrasound-assisted conjugates. This study highlights how ultrasound can intensify the wet-heating glycation of proteins to modify their structure, consequently improving their techno-functional properties to provide a novel plant-based protein ingredient for various food applications.
期刊介绍:
Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels.
Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.