Correlation between various loads and apoptosis in medial platform chondrocytes in knee varus deformity

IF 1.4 3区 医学 Q4 ENGINEERING, BIOMEDICAL
Hongjie Zhang , Zehua Jiang , Ziyang Liu , Zhixiong Hong , Mengqiang Tian , Rusen Zhu
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引用次数: 0

Abstract

Background

This study aimed to systematically investigate how different degrees of mechanical loading caused by varying varus angles affect stress distribution and chondrocyte apoptosis in the medial tibial plateau of the knee joint. Specifically, it integrates finite-element analysis to simulate biomechanical stress patterns and evaluates the molecular responses (Piezo1, Bax, Bcl-2, and caspase-3 expressions) to elucidate the mechanobiological interplay contributing to cartilage degeneration.

Methods

Four knee models with varus angles (6°, 9°, 12°, and 15°) were constructed from computed tomography images. Finite-element analysis was used to evaluate stress distribution and von Mises stress peaks on the medial tibial platform cartilage. Material properties of biological tissues were included. Clinical samples of corresponding varus angles were analyzed for Piezo1, Bax, Bcl-2, and caspase-3 expression using immunofluorescence and histochemistry.

Findings

The von Mises stress peak contact area of the medial tibial plateau cartilage significantly decreased with increasing varus deformity (p < 0.05), and Piezo1 expression increased with stress load. Elevated Piezo1 expression was associated with significantly higher levels of Bax, Bcl-2, and caspase-3 (p < 0.05).

Interpretation

Pathological mechanical loading accelerates chondrocyte apoptosis via the endogenous apoptotic pathway, promoting the progression of knee osteoarthritis. These findings highlight Piezo1 as a potential therapeutic target for managing stress-induced cartilage degeneration.
膝关节内翻畸形中内侧平台软骨细胞凋亡与负荷的关系
本研究旨在系统研究不同内翻角度引起的不同程度的机械负荷对膝关节内侧胫骨平台应力分布和软骨细胞凋亡的影响。具体来说,它整合了有限元分析来模拟生物力学应力模式,并评估分子反应(Piezo1, Bax, Bcl-2和caspase-3表达),以阐明导致软骨退变的力学生物学相互作用。方法利用计算机断层图像构建4个膝内翻角(6°、9°、12°、15°)模型。采用有限元分析评估胫骨内侧平台软骨的应力分布和von Mises应力峰值。包括生物组织的材料特性。采用免疫荧光和组织化学方法分析相应内翻角临床标本中Piezo1、Bax、Bcl-2和caspase-3的表达。结果胫骨平台内侧软骨von Mises应力峰值接触面积随内翻畸形的增加而显著降低(p < 0.05),且Piezo1表达随应力负荷的增加而升高。Piezo1表达升高与Bax、Bcl-2和caspase-3水平显著升高相关(p < 0.05)。病理机械负荷通过内源性凋亡途径加速软骨细胞凋亡,促进膝关节骨关节炎的进展。这些发现突出了Piezo1作为管理应力诱导的软骨变性的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical Biomechanics
Clinical Biomechanics 医学-工程:生物医学
CiteScore
3.30
自引率
5.60%
发文量
189
审稿时长
12.3 weeks
期刊介绍: Clinical Biomechanics is an international multidisciplinary journal of biomechanics with a focus on medical and clinical applications of new knowledge in the field. The science of biomechanics helps explain the causes of cell, tissue, organ and body system disorders, and supports clinicians in the diagnosis, prognosis and evaluation of treatment methods and technologies. Clinical Biomechanics aims to strengthen the links between laboratory and clinic by publishing cutting-edge biomechanics research which helps to explain the causes of injury and disease, and which provides evidence contributing to improved clinical management. A rigorous peer review system is employed and every attempt is made to process and publish top-quality papers promptly. Clinical Biomechanics explores all facets of body system, organ, tissue and cell biomechanics, with an emphasis on medical and clinical applications of the basic science aspects. The role of basic science is therefore recognized in a medical or clinical context. The readership of the journal closely reflects its multi-disciplinary contents, being a balance of scientists, engineers and clinicians. The contents are in the form of research papers, brief reports, review papers and correspondence, whilst special interest issues and supplements are published from time to time. Disciplines covered include biomechanics and mechanobiology at all scales, bioengineering and use of tissue engineering and biomaterials for clinical applications, biophysics, as well as biomechanical aspects of medical robotics, ergonomics, physical and occupational therapeutics and rehabilitation.
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