Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity

Abstract

T lymphocytes are key contributors to the adaptive immune system. During development, tightly regulated T cell receptor (TCR) gene rearrangement determines antigen specificity and maturation into distinct lineages with pro- or anti-inflammatory functions. From two male donors, we generated four hiPSC lines from isolated T cell lineages (CD4+ conventional helper, CD4+ regulatory, CD8+ cytotoxic). Two lines harbor genetically pre-rearranged TCRs specific to an allogeneic cell line. All lines were generated by integration-free reprogramming using Sendai virus and underwent characterization and quality control. They represent a valuable platform to investigate how a rearranged and pre-selected TCR influences lymphoid differentiation and function.