Effect of experimentally induced sepsis on the kidney and renal artery of adult male albino rats and the role of Boswellia serrata extract

Q3 Medicine

Morphologie Pub Date : 2025-08-27 DOI:10.1016/j.morpho.2025.101065

M.A. Al-Gholam , N.M. Issa , A.S.A. Alafify

{"title":"Effect of experimentally induced sepsis on the kidney and renal artery of adult male albino rats and the role of Boswellia serrata extract","authors":"M.A. Al-Gholam , N.M. Issa , A.S.A. Alafify","doi":"10.1016/j.morpho.2025.101065","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Sepsis can be cured with effective antibiotics against susceptible organisms if treatment is provided early and promptly. As a result of antibiotic resistance and chemotherapeutic agents’ side effects, the use of these medications is restricted. Thus, the search for novel therapeutic targets and studies on the pathogenesis of sepsis has increased. In this study, we aimed to examine the treatment effects of <em>Boswellia serrata extract</em> (<em>BSE</em>) pre- or pre and post-treatment after sepsis induction.</div></div><div><h3>Material and methods</h3><div>adult male albino rats were assigned into 5 groups: control, <em>BSE</em>, septic, septic pre-treated, or septic pre and post-treated by <em>BSE</em>. Sepsis induction was performed by modified cecal slurry and 500mg/kg/oral of <em>BSE</em> was administered.</div></div><div><h3>Results</h3><div>It was found that the elevated serum urea and creatinine in the septic group were ameliorated by pre- or pre and post-treatment with <em>BSE</em>. Parallel histopathological examinations revealed that <em>BSE</em> ameliorated the pathological changes of the kidney and renal artery including shrinkage of capillary tufts, widening of Bowman's space, focal tubular dilatation and appearance of casts inside the tubules, desquamation of endothelium, thickening of tunica media decreasing the diameter of the lumen of the renal artery compared to the septic group. Periodic acid Schiff staining increased while collagen fibers decreased in <em>BSE</em>-treated groups (<em>P</em>  <0.001) and E-cadherin expression was improved in the <em>BSE</em>-treated groups (<em>P</em>  <!-->0.001).</div></div><div><h3>Conclusion</h3><div>These data indicate that <em>BSE</em> minimizes the lesions of sepsis-induced kidney injury and could be useful in improving the pathological alterations of parenchymal organs associated with sepsis.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"109 367","pages":"Article 101065"},"PeriodicalIF":0.0000,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Morphologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1286011525001171","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Sepsis can be cured with effective antibiotics against susceptible organisms if treatment is provided early and promptly. As a result of antibiotic resistance and chemotherapeutic agents’ side effects, the use of these medications is restricted. Thus, the search for novel therapeutic targets and studies on the pathogenesis of sepsis has increased. In this study, we aimed to examine the treatment effects of Boswellia serrata extract (BSE) pre- or pre and post-treatment after sepsis induction.

Material and methods

adult male albino rats were assigned into 5 groups: control, BSE, septic, septic pre-treated, or septic pre and post-treated by BSE. Sepsis induction was performed by modified cecal slurry and 500 mg/kg/oral of BSE was administered.

Results

It was found that the elevated serum urea and creatinine in the septic group were ameliorated by pre- or pre and post-treatment with BSE. Parallel histopathological examinations revealed that BSE ameliorated the pathological changes of the kidney and renal artery including shrinkage of capillary tufts, widening of Bowman's space, focal tubular dilatation and appearance of casts inside the tubules, desquamation of endothelium, thickening of tunica media decreasing the diameter of the lumen of the renal artery compared to the septic group. Periodic acid Schiff staining increased while collagen fibers decreased in BSE-treated groups (P < 0.001). Additionally, Cluster of Differentiation 15 (CD15) and the paired box (PAX) expression were downregulated (P < 0.001) and E-cadherin expression was improved in the BSE-treated groups (P < 0.001).

Conclusion

These data indicate that BSE minimizes the lesions of sepsis-induced kidney injury and could be useful in improving the pathological alterations of parenchymal organs associated with sepsis.

查看原文本刊更多论文

实验性脓毒症对成年雄性白化大鼠肾脏和肾动脉的影响及锯齿乳香提取物的作用

如果及早和及时提供治疗，对易感生物有效的抗生素可以治愈败血症。由于抗生素耐药性和化疗药物的副作用，这些药物的使用受到限制。因此，寻找新的治疗靶点和对脓毒症发病机制的研究已经增加。在本研究中，我们旨在研究Boswellia serrata extract （BSE）在脓毒症诱导前后的治疗效果。材料与方法将成年雄性白化大鼠分为5组：对照组、BSE组、脓毒症组、脓毒症预处理组、脓毒症前后处理组。改良盲肠浆液诱导脓毒症，给予BSE 500 mg/kg/口服。结果脓毒症患者的血清尿素和肌酐升高情况在疯牛病治疗前或前后均有改善。平行组织病理学检查显示，与脓毒症组相比，BSE改善了肾脏和肾动脉的病理改变，包括毛细血管丛缩小，鲍曼间隙变宽，局灶性小管扩张，小管内出现铸型，内皮脱屑，中膜增厚，肾动脉管腔直径减小。bse处理组周期性酸性席夫染色增加，胶原纤维减少（P < 0.001）。此外，bse处理组CD15和PAX表达下调（P < 0.001）， E-cadherin表达提高（P < 0.001）。结论疯牛病可使脓毒症所致肾损伤的损害最小化，并可改善脓毒症相关肾实质器官的病理改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Morphologie Medicine-Anatomy

CiteScore

2.30

自引率

0.00%

发文量

150

审稿时长

25 days

期刊介绍： Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.