Cas13-mediated liquid biopsy: A comprehensive review of cancer diagnostic applications

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-08-21 DOI:10.1016/j.trac.2025.118411

Menglu Gao , Si Wu , Xin Hu , Lihan Zhu , Yue Hu , Xin He , Jiurong He , Rong Yuan , Yinhua Zhang , Cheng Liu , Fu-Bing Wang

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引用次数: 0

Abstract

Over the past decade, CRISPR-based molecular editing technologies have found extensive applications across various disease domains. Among them, CRISPR Cas13, a significant member of the CRISPR family, has been employed in a non-invasive diagnostic method that analyzes circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), microRNAs (miRNAs), and extracellular vehicles (EVs) isolated from biological fluids such as blood, urine, saliva, cerebrospinal fluid, and pleural effusion. This has established CRISPR Cas13 as a crucial tool in tumor liquid biopsies. We have reviewed the structural compositions of different CRISPR Cas13 members and optimization strategies for the Cas13 reaction system, including guiding RNA (gRNA) design, integration of nanomaterials, electrochemical sensors, and microfluidic chips. By examining Cas13 applications in both in vivo and in vitro tumor detection, we delve into the innovative and practical potential of these approaches and highlight their promise as transformative tools in cancer diagnostics.

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cas13介导的液体活检：癌症诊断应用的全面回顾

在过去的十年中，基于crispr的分子编辑技术在各种疾病领域得到了广泛的应用。其中，CRISPR Cas13作为CRISPR家族的重要成员，已被用于分析血液、尿液、唾液、脑脊液和胸腔积液等生物体液中分离的循环肿瘤DNA （ctDNA）、循环肿瘤细胞（CTCs）、microRNAs （miRNAs）和细胞外载体（ev）的无创诊断方法。这使得CRISPR Cas13成为肿瘤液体活检的关键工具。我们综述了不同CRISPR Cas13成员的结构组成和Cas13反应体系的优化策略，包括引导RNA （gRNA）设计、纳米材料集成、电化学传感器和微流控芯片。通过研究Cas13在体内和体外肿瘤检测中的应用，我们深入研究了这些方法的创新和实践潜力，并强调了它们作为癌症诊断变革工具的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.