Poplar–wheat intercropping and fertilizer application significantly improve soil bacterial community characteristic and nutrient contents

IF 5 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology Pub Date : 2025-08-28 DOI:10.1016/j.apsoil.2025.106415

Cheng Xu , Xiaopeng Liu , Zhuangzhuang Qian , Tao Yang , Bo Wang , Xiaomin Ge , Luozhong Tang

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引用次数: 0

Abstract

Intercropping can significantly influence soil microbial diversity and nutrient content. However, the effects of intercropping with different fertilizer application amounts on soil bacterial community characteristic and nutrient contents remain unclear. A pot experiment with three planting patterns (P (poplar monoculture; Populus deltoides), W (wheat monoculture; Triticum aestivum) and PW (poplar–wheat intercropping)) and three fertilization rates of 0 g, 5 g, and 10 g compound fertilizer (10 kg soil per pot; N:P₂O₅:K₂O ratio of 15 %:15 %:15 %) was performed. Soil properties and bacterial community were analyzed under different treatments. PW pattern significantly increased SOC (soil organic carbon), TN (total nitrogen), SMBC (soil microbial biomass carbon), SOC:TN, SMBC:SMBN (soil microbial biomass nitrogen), and qMBC (microbial biomass carbon quotient) and greater UR (urease) and SU (sucrase) activities than W and P patterns did, with the highest values occurring in 10 g fertilizer treatment (P < 0.05). The shifts in bacterial community composition associated with PW (i.e., increases in Pseudomonadota, Acidobacteriota, and Pseudarthrobacter abundances) for bacteria were positively associated with SOC, TN, SMBC, SMBN, qMBC, qMBN, UR and SU (P < 0.05). Soil water content was the dominant factor affecting soil bacterial community by mantel analysis. Intercropping significantly increased soil bacteria related to nutrient transformation. Fertilizer application significantly affected soil bacterial diversity; however, planting pattern significantly mainly affected soil bacterial community composition. Besides, fertilizer application can reduce the differences in bacterial diversity among planting patterns. In conclusion, these findings revealed the mechanism by which intercropping and fertilizer application improve soil bacterial community characteristic and nutrient content. Our study serves as a reference for enhancing soil quality through land management practices.

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杨麦间作和施肥显著改善了土壤细菌群落特征和养分含量

间作对土壤微生物多样性和养分含量有显著影响。然而，不同施肥量间作对土壤细菌群落特征和养分含量的影响尚不清楚。盆栽试验采用3种种植模式(P（杨树单作、三角杨树）、W（小麦单作、小麦小麦）和PW（杨树-小麦间作），3种施肥水平分别为0、5、10 g复合肥（每盆土壤10 kg， N:P2O5:K2O比例为15%:15%:15%）。分析了不同处理下土壤性质和细菌群落。与W和P模式相比，PW模式显著提高了土壤有机碳（SOC）、总氮（TN）、土壤微生物生物量碳（SMBC）、SOC:TN、SMBC:SMBN（土壤微生物生物量氮）和微生物生物量碳商（qMBC），提高了脲酶（UR）和蔗糖酶（SU）活性，且以10 g肥处理最高（P < 0.05）。与PW相关的细菌群落组成变化（即假单胞菌、酸杆菌和假节杆菌丰度的增加）与细菌的SOC、TN、SMBC、SMBN、qMBC、qMBN、UR和SU呈正相关（P < 0.05）。土壤含水量是影响土壤细菌群落的主要因素。间作显著增加了与养分转化有关的土壤细菌。施肥对土壤细菌多样性影响显著；而种植方式对土壤细菌群落组成的影响显著。此外，施肥可以减小不同种植方式间细菌多样性的差异。综上所述，这些结果揭示了间作和施肥改善土壤细菌群落特征和养分含量的机制。我们的研究为通过土地管理措施改善土壤质量提供了参考。

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来源期刊

Applied Soil Ecology 农林科学-土壤科学

CiteScore

9.70

自引率

4.20%

发文量

363

审稿时长

5.3 months

期刊介绍： Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.