An LED-based multi-sample absorbance spectrophotometer for chemistry and biochemistry

IF 2.1 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

HardwareX Pub Date : 2025-08-21 DOI:10.1016/j.ohx.2025.e00690

David P. Goldenberg

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引用次数: 0

Abstract

The instrument described in this article measures the absorbance of visible light (with wavelengths in the range of approximately 400 to 650 nm) by liquid samples, a method widely used for determining solute concentrations. To minimize the cost of the instrument, interchangeable light-emitting diodes (LEDs) are used as light sources. Transmitted light is detected using a photodiode device and the signals are processed using an Arduino microprocessor board. Measured absorbances are displayed on an LCD panel and can be transferred to another device via a USB interface. The instrument has measuring positions for up to six samples, each with a separate LED and detector, making it particularly well suited for parallel kinetic experiments with multiple samples. Because the spectrophotometer was designed with undergraduate laboratory courses in mind, it has a modular construction that allows for easy assembly and disassembly, so that students can be given an opportunity to assemble the instrument themselves. The device has a power requirement of only 0.4 W from a 5 V USB supply, making it practical for field studies or other applications where access to electric power is limited.

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一种基于led的化学和生物化学多样品吸收分光光度计

本文描述的仪器测量液体样品可见光（波长范围约为400至650 nm）的吸光度，这是一种广泛用于测定溶质浓度的方法。为了将仪器的成本降至最低，使用可互换发光二极管（led）作为光源。通过光电二极管设备检测透射光，并使用Arduino微处理器板处理信号。测量的吸光度显示在LCD面板上，可以通过USB接口传输到另一个设备。该仪器有多达六个样品的测量位置，每个都有一个单独的LED和检测器，使其特别适合于多个样品的平行动力学实验。由于分光光度计是根据本科实验课程设计的，因此它具有模块化结构，易于组装和拆卸，因此学生可以有机会自己组装仪器。该设备的功率要求仅为0.4 W，来自5 V USB电源，使其适用于现场研究或其他电力有限的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.