A long-term universal impedance flow cytometry platform empowered by adaptive channel height and real-time clogging-release strategy

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-08-26 DOI:10.1039/d5lc00673b
Trisna Julian, Tao Tang, Naomi Tanga, Yang Yang, Yoichiroh Hosokawa, Yaxiaer Yalikun
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引用次数: 0

Abstract

Impedance flow cytometry is a widely used label-free technique for single-cell analysis; however, its limited sensitivity and lack of universality have hindered its ability to replace conventional flow cytometry. In this study, we propose an adaptive microfluidic channel platform that dynamically adjusts the channel height to improve both measurement performance and system versatility. We found that reducing the channel height by one-third effectively decreases the distance between particles and the sensing electrodes, resulting in an average 3.2-fold amplification of the impedance signal. This approach also reduces signal variability by half, thereby enhancing measurement precision. Enhanced particle discrimination was demonstrated using a mixture of yeast cells and 6 μm beads, while robust cell phenotyping was achieved across multiple cell lines, including A549, C6, and NIH/3T3. By integrating this adaptive channel with an object detection algorithm, we successfully created a self-optimizing system that utilizes intentional, temporary clogging as a strategy to regulate channel height. These findings underscore the potential for a universal, high-performance impedance flow cytometry platform that simple, clog-resistant, and adaptable for a wide range of biomedical applications.

Abstract Image

一个长期的通用阻抗流式细胞仪平台,具有自适应通道高度和实时阻塞释放策略
阻抗流式细胞术是一种广泛应用于单细胞分析的无标记技术;然而,其有限的灵敏度和缺乏普遍性阻碍了其取代传统流式细胞术的能力。在这项研究中,我们提出了一个自适应微流体通道平台,动态调整通道高度,以提高测量性能和系统的通用性。我们发现,将通道高度降低三分之一可以有效地减少粒子与传感电极之间的距离,从而使阻抗信号平均放大3.2倍。该方法还将信号变异性降低了一半,从而提高了测量精度。使用酵母细胞和6 μm微珠的混合物增强了颗粒识别,同时在多个细胞系(包括A549, C6和NIH/3T3)中实现了稳健的细胞表型。通过将这种自适应通道与目标检测算法相结合,我们成功地创建了一个自我优化系统,该系统利用故意的、暂时的阻塞作为调节通道高度的策略。这些发现强调了一种通用的、高性能的阻抗流式细胞仪平台的潜力,这种平台简单、耐堵塞,适用于广泛的生物医学应用。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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