Impaired Treg‐Mediated Immune Regulation in Peri‐Implantitis Lesions and Implant Loss: Insights From Histological and Molecular Analyses

IF 6.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2025-08-26 DOI:10.1111/jcpe.70026

Emilio A. Cafferata, Ausra Ramanauskaite, Puria Parvini, Clemens Raabe, Eva Dohle, Shahram Ghanaati, Frank Schwarz

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Abstract

AimTo evaluate the T regulatory lymphocyte (Treg) profile and its potential contribution to peri‐implant tissue destruction during peri‐implantitis (PI).MethodsPI granulation tissue and crevicular fluid collected during PI surgical (PI group, n = 23) and explantation (PI‐X group, n = 23) therapy, with peri‐implant healthy tissue from second‐stage surgery (H group, n = 20) as controls, were analysed. The inflammatory infiltrate was characterised by H&E staining. The relative expression of Treg‐associated transcription factors and cytokines was assessed by RT‐qPCR. Forkhead box P3 (FOXP3) and neuropilin (NPR)‐1 were detected by immunohistochemistry, and interleukin (IL)‐10, TGF‐β1 and IL‐35 by ELISA. The clinical parameters, namely probing depth (PD), bleeding on probing (BOP) and vertical defect depth (VDD), were also recorded.ResultsPI and PI‐X lesions showed up‐regulation of FOXP3, HELIOS and IL35B and down‐regulation of NRP1 and TGFβ1 mRNA expression, compared to H tissue (p < 0.05). Significantly more FOXP3+ cells and significantly less NRP‐1+ area were detected in PI and PI‐X lesions (p < 0.05). IL‐35 levels were up‐regulated, whereas TGF‐β1 levels were down‐regulated in PI and PI‐X lesions, compared to H samples (p < 0.05). PD and VDD were significantly correlated with the down‐regulation of FOXP3 and NRP‐1 (p < 0.05).ConclusionsTreg dysfunction and altered cytokine profiles in PI are associated with inflammation and clinical disease severity.

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Treg介导的免疫调节在种植体周围病变和种植体损失中的受损：来自组织学和分子分析的见解

目的评估T调节性淋巴细胞（Treg）谱及其在种植体周围炎（PI）期间对种植体周围组织破坏的潜在贡献。方法分析PI手术（PI组，n = 23）和移植（PI - X组，n = 23）治疗期间收集的肉芽组织和沟液，并以第二阶段手术（H组，n = 20）的种植周健康组织为对照。H&；E染色表征炎症浸润。RT - qPCR检测Treg相关转录因子和细胞因子的相对表达。免疫组织化学检测叉头盒P3 （FOXP3）和神经磷脂（NPR）‐1，ELISA检测白细胞介素（IL）‐10、TGF‐β1和IL‐35。记录临床参数，即探探深度（PD）、探探出血（BOP）和垂直缺损深度（VDD）。结果spi和PI - X病变FOXP3、HELIOS和IL35B表达上调，NRP1和tgf - β1 mRNA表达下调（p < 0.05）。PI和PI‐X病变中FOXP3+细胞明显增多，NRP‐1+细胞面积明显减少（p < 0.05）。与H样品相比，PI和PI - X病变中IL - 35水平上调，而TGF - β1水平下调（p < 0.05）。PD和VDD与FOXP3和NRP - 1的下调显著相关（p < 0.05）。结论PI的g功能障碍和细胞因子谱改变与炎症和临床疾病严重程度相关。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.