Cerebrospinal Fluid Cytokine and Chemokine Analysis in Dogs With Meningoencephalitis of Unknown Origin or Idiopathic Epilepsy

IF 2.2 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2025-08-26 DOI:10.1111/jvim.70229

Michael J. Byron, Alison L. Parker, Stephen Parry, Yael Merbl

{"title":"Cerebrospinal Fluid Cytokine and Chemokine Analysis in Dogs With Meningoencephalitis of Unknown Origin or Idiopathic Epilepsy","authors":"Michael J. Byron, Alison L. Parker, Stephen Parry, Yael Merbl","doi":"10.1111/jvim.70229","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Current diagnosis of brain disease in dogs is dependent on imaging and cerebrospinal fluid (CSF) analysis, including total nucleated cell counts and albumin concentrations.</p>\n </section>\n \n <section>\n \n <h3> Hypothesis/Objectives</h3>\n \n <p>To determine whether multiplex cytokine/chemokine (Ct/Cm) analysis can differentiate among dogs with meningoencephalitis of unknown origin (MUE), idiopathic epilepsy (IE), and brain neoplasia.</p>\n </section>\n \n <section>\n \n <h3> Animals</h3>\n \n <p>Client owned dogs diagnosed with brain disease with MRI and CSF diagnostics. Groups included 18 dogs with a diagnosis of MUE, 21 dogs with IE, and 7 dogs with brain tumors.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A retrospective observational study; a multiplex immunoassay was utilized to measure CSF concentrations for the following: Interleukin (IL)-2, IL-6, IL-7, IL-8, IL-10, IL-15, and IL-18, granulocyte macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-γ), keratinocyte chemoattractant (KC)-like protein, IFN-γ-inducible protein-10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor alpha (TNF-α).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Several Ct/Cm were detected only in MUE cases: GM-CSF (9/18), IFN-γ (13/18), IL-2 (8/18), IL-15 (4/18), and TNF-α (11/18). Other Ct/Cm concentrations were significantly higher in MUE cases (IL-8: median 101 pg/mL, range 144, <i>p</i> = 0.019; IL-18: median 3 pg/mL, range 0.52, <i>p</i> < 0.001; MCP-1: median 814 pg/mL, range 1319, <i>p</i> = 0.004; and IL-6: median 5 pg/mL, range 16, <i>p</i> < 0.001) compared to epilepsy and neoplasia.</p>\n </section>\n \n <section>\n \n <h3> Conclusions and Clinical Importance</h3>\n \n <p>IFN-γ, TNF-α, GM-CSF, IL-2, and IL-15 might be specific markers of MUE in canine CSF and could be potentially useful biomarkers in the diagnosis of MUE.</p>\n </section>\n </div>","PeriodicalId":49958,"journal":{"name":"Journal of Veterinary Internal Medicine","volume":"39 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70229","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Internal Medicine","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jvim.70229","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Current diagnosis of brain disease in dogs is dependent on imaging and cerebrospinal fluid (CSF) analysis, including total nucleated cell counts and albumin concentrations.

Hypothesis/Objectives

To determine whether multiplex cytokine/chemokine (Ct/Cm) analysis can differentiate among dogs with meningoencephalitis of unknown origin (MUE), idiopathic epilepsy (IE), and brain neoplasia.

Animals

Client owned dogs diagnosed with brain disease with MRI and CSF diagnostics. Groups included 18 dogs with a diagnosis of MUE, 21 dogs with IE, and 7 dogs with brain tumors.

Methods

A retrospective observational study; a multiplex immunoassay was utilized to measure CSF concentrations for the following: Interleukin (IL)-2, IL-6, IL-7, IL-8, IL-10, IL-15, and IL-18, granulocyte macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-γ), keratinocyte chemoattractant (KC)-like protein, IFN-γ-inducible protein-10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor alpha (TNF-α).

Results

Several Ct/Cm were detected only in MUE cases: GM-CSF (9/18), IFN-γ (13/18), IL-2 (8/18), IL-15 (4/18), and TNF-α (11/18). Other Ct/Cm concentrations were significantly higher in MUE cases (IL-8: median 101 pg/mL, range 144, p = 0.019; IL-18: median 3 pg/mL, range 0.52, p < 0.001; MCP-1: median 814 pg/mL, range 1319, p = 0.004; and IL-6: median 5 pg/mL, range 16, p < 0.001) compared to epilepsy and neoplasia.

Conclusions and Clinical Importance

IFN-γ, TNF-α, GM-CSF, IL-2, and IL-15 might be specific markers of MUE in canine CSF and could be potentially useful biomarkers in the diagnosis of MUE.

Abstract Image

查看原文本刊更多论文

不明原因脑膜脑炎或特发性癫痫犬脑脊液细胞因子和趋化因子分析

目前犬脑疾病的诊断依赖于影像学和脑脊液（CSF）分析，包括总有核细胞计数和白蛋白浓度。假设/目的探讨多元细胞因子/趋化因子（Ct/Cm）分析是否能鉴别不明原因脑膜脑炎（MUE）、特发性癫痫（IE）和脑瘤。客户拥有的狗经MRI和CSF诊断为脑部疾病。各组包括18只诊断为MUE的狗，21只诊断为IE的狗，7只诊断为脑肿瘤的狗。方法回顾性观察研究；采用多元免疫分析法测定CSF中以下物质的浓度：白细胞介素(IL)-2、IL-6、IL-7、IL-8、IL-10、IL-15和IL-18、粒细胞巨噬细胞集落刺激因子（GM-CSF）、干扰素γ (IFN-γ)、角质细胞趋化剂（KC）样蛋白、IFN-γ诱导蛋白-10 （IP-10）、单核细胞趋化蛋白1 （MCP-1）和肿瘤坏死因子α (TNF-α)。结果仅在MUE病例中检测到几种Ct/Cm: GM-CSF（9/18）、IFN-γ(13/18)、IL-2（8/18）、IL-15（4/18）和TNF-α(11/18)。与癫痫和肿瘤相比，MUE病例的其他Ct/Cm浓度显著升高（IL-8：中位数101 pg/mL，范围144，p = 0.019； IL-18：中位数3 pg/mL，范围0.52,p < 0.001； MCP-1：中位数814 pg/mL，范围1319，p = 0.004； IL-6：中位数5 pg/mL，范围16，p < 0.001）。IFN-γ、TNF-α、GM-CSF、IL-2和IL-15可能是犬脑脊液中MUE的特异性标志物，可能是诊断MUE的潜在有用的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.