Multifunctional hydroxyapatite-carbon dot nanocomposites grafted with polyglycerol: synthesis, characterization, and application in self-targeted cell imaging and drug delivery

IF 2.6 4区材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of Nanoparticle Research Pub Date : 2025-08-26 DOI:10.1007/s11051-025-06414-y

Peijian Yang, Jie Wang, Haiyun Zhai, Xiulan Cai

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引用次数: 0

Abstract

In this project, a novel ultrasound-mediated synthesis route was firstly developed to prepare carbon dots (CDs) decorated hydroxyapatite (HAp) targeted fluorescent nanocomposites (HAp-CDs) under low concentration of folic acid. Folic acid not only served as carbon source to form fluorescent CDs but also as targeted molecules to specifically recognize HeLa human cervical cancer cells. Furthermore, the HAp surface of HAp-CDs nanocomposites was successfully grafted with hyperbranched polyglycerol (HAp-CDs-PG) through surface-initiated ring-opening polymerization of glycidol. The grafted PG layer not only tremendously enhanced the aqueous dispersibility and stability of HAp-CDs nanocomposites, but also effectively inhibited non-specific cellular uptake of HAp-CDs-PG. Finally, the folate residues on HAp-CDs-PG achieved targeted uptake by recognizing folate receptors on Hela cells, while HAp-CDs-PG was mainly located in the cytosol of HeLa cells after being uptaken. Due to its good biocompatibility as determined by Cell Counting Kit-8 (CCK-8), HAp-CDs-PG was used to load anticancer drug doxorubicin (Dox) and efficiently delivered into targeted cells by folate receptor-mediated endocytosis, leading to an enhanced therapeutic efficacy of Dox. Thus, the HAp-CDs-PG nanocomposites could potentially be used for simultaneous cancer-targeted drug delivery and self-targeted cell imaging.

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聚甘油接枝多功能羟基磷灰石-碳点纳米复合材料：合成、表征及其在自体靶向细胞成像和药物传递中的应用

本课题首次开发了一种在低浓度叶酸条件下制备碳点修饰羟基磷灰石（HAp）靶向荧光纳米复合材料（HAp-CDs）的超声介导合成新途径。叶酸不仅可以作为碳源形成荧光CDs，还可以作为靶向分子特异性识别HeLa人宫颈癌细胞。此外，通过表面引发的甘油开环聚合，在HAp- cds纳米复合材料的HAp表面成功接枝了超支化聚甘油（HAp- cds - pg）。接枝的PG层不仅极大地提高了HAp-CDs纳米复合材料的水分散性和稳定性，而且有效地抑制了HAp-CDs-PG的非特异性细胞摄取。最后，HAp-CDs-PG上的叶酸残基通过识别Hela细胞上的叶酸受体实现了靶向摄取，而HAp-CDs-PG被摄取后主要定位于Hela细胞的细胞质中。通过细胞计数试剂盒-8 （CCK-8）检测，HAp-CDs-PG具有良好的生物相容性，可用于装载抗癌药物阿霉素（Dox），并通过叶酸受体介导的内吞作用有效地递送到靶细胞中，从而提高阿霉素的治疗效果。因此，HAp-CDs-PG纳米复合材料有可能同时用于癌症靶向药物递送和自我靶向细胞成像。

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来源期刊

Journal of Nanoparticle Research 工程技术-材料科学：综合

CiteScore

4.40

自引率

4.00%

发文量

198

审稿时长

3.9 months

期刊介绍： The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.