MXene/Graphene Nanoribbons Composite Materials for High-Sensitivity Enzyme-Free Electrochemiluminescent Detection of L-Lactic acid

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2025-08-24 DOI:10.1016/j.snr.2025.100379

Haicheng Song , Xiaoqian Zhou , Lixing Lin , Xinying Zhang , Yingying Chen , Zhenzhen Li , Lingyan Feng

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引用次数: 0

Abstract

Lactic acid level is a crucial biochemical indicator in the field of cancer diagnosis, anti-tumor therapy and other related biomedical areas. However, currently the method for detecting lactic acid is almost enzyme-based biosensors, while enzyme-free alternatives remain underexplored. In this work, we constructed a high-performance electrochemiluminescence (ECL) sensors based on Ti₃C₂ MXene/graphene nanoribbons (MXene/GNRs) composite for the enzyme-free detection of L-lactic acid. Meanwhile, we functionalize MXene/GNRs composite with the L-lactic acid aptamer through physical and chemical interactions, and with the addition of the target L-Lactic acid, the aptamer was detached from the surface of the material, thus, the biosensor was constructed with an "on-off-on" ECL signal. The biosensor designed for this work demonstrated a detection range of L-Lactic acid from 500 nM to 10 mM, and a low detection limit of 309 nM. This work exhibits superior detection performance in complex environments, and also presents a promising enzyme-free approach for L-lactic acid monitoring in biomedical applications.

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MXene/石墨烯纳米带复合材料高灵敏度无酶电化学发光检测l -乳酸

乳酸水平是肿瘤诊断、抗肿瘤治疗等相关生物医学领域的重要生化指标。然而，目前检测乳酸的方法几乎是基于酶的生物传感器，而无酶的替代品仍未得到充分的探索。在这项工作中，我们构建了一种基于Ti3C2 MXene/石墨烯纳米带（MXene/GNRs）复合材料的高性能电化学发光（ECL）传感器，用于无酶检测l -乳酸。同时，我们通过l -乳酸适配体与MXene/GNRs复合材料的物理化学相互作用实现功能化，随着目标l -乳酸的加入，适配体从材料表面分离，从而构建了具有“on-off-on”ECL信号的生物传感器。该传感器对l -乳酸的检测范围为500 nM ~ 10 mM，最低检测限为309 nM。这项工作在复杂环境中表现出优越的检测性能，也为生物医学应用中的l -乳酸监测提供了一种有前途的无酶方法。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.