A workflow for sourcing and characterizing wheat field microorganisms for the biocontrol of Fusarium head blight

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2025-07-21 DOI:10.1016/j.biocontrol.2025.105856

Toan Bao Hung Nguyen, Flora Pensec, Jérôme Mounier, Monika Coton, Amandine Henri-Sanvoisin, Massilia Mouzaoui, Adeline Picot

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引用次数: 0

Abstract

Fusarium head blight (FHB), caused by numerous Fusarium species, is a major fungal disease affecting wheat and other food staples. Biological control agents (BCAs) are promising alternatives to synthetic fungicides for integrated disease management. In the present study, a workflow was developed to isolate and screen wheat field-associated microorganisms for their antagonistic activities against FHB causal agents, by taking into account both intra- and interspecific diversity of pathogen complexes. Non-selective and selective isolation methods were used to collect 1231 bacteria, 365 molds, and 39 yeasts from wheat grains, maize residues, and soil samples. From this collection, microbial antagonistic activities were first determined against a Fusarium graminearum strain, followed by taxonomic identification of positive isolates. Their activity spectrum was also determined against additional Fusarium graminearum strains, as well as Fusarium avenaceum and Fusarium poae. Then, screening of the ten most effective isolates (4 bacteria, 1 yeast-like, and 5 molds: Rahnella aceris, Streptomyces sp., 2 Pantoea agglomerans isolates, Cistella sp., Trichoderma paraviridescens, Trichoderma gamsii, Epicoccum layuense, Podila minutissima, and Penicillium virgatum) on surface-disinfected grains confirmed their ability to control or delay pathogen growth. Significant variations in BCA antagonistic activities were observed among isolates across different Fusarium species and/or strains, which reinforced the importance of including intra- and interspecific pathogen diversity during the screening steps. One T. gamsii isolate emerged as the most promising candidate, achieving complete inhibition of Fusarium growth (100%) and preventing or dramatically reducing mycotoxin production (up to 100% reduction of deoxynivalenol production). This study highlights that wheat fields serve as a valuable reservoir for potential BCAs against FHB control.

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用于小麦枯萎病生物防治的田间微生物来源和鉴定工作流程

镰刀菌头疫病（Fusarium head blight， FHB）是一种由多种镰刀菌引起的主要真菌疾病，影响小麦和其他主要粮食。生物防治剂（bca）是替代合成杀菌剂进行疾病综合治理的一种有前景的方法。在本研究中，通过考虑病原体复合物的种内和种间多样性，开发了一种工作流程来分离和筛选麦田相关微生物对FHB致病因子的拮抗活性。采用非选择性和选择性分离方法从小麦、玉米秸秆和土壤样品中分离到1231种细菌、365种霉菌和39种酵母。从这些收集物中，首先确定了对一株谷物镰刀菌的微生物拮抗活性，然后对阳性分离物进行了分类鉴定。测定了它们对其他谷物镰刀菌、燕麦镰刀菌和poae镰刀菌的活性谱。然后，在表面消毒的谷物上筛选10种最有效的分离物（4种细菌，1种酵母样菌和5种霉菌：酸Rahnella aceris， Streptomyces sp.， 2种Pantoea agglomerans分离物，citella sp.， paraviri下降木霉，gamsii木霉，表皮霉，Podila minutissima和virgatum Penicillium），证实它们具有控制或延缓病原体生长的能力。在不同镰刀菌种类和/或菌株的分离物中观察到BCA拮抗活性的显著差异，这加强了在筛选步骤中包括种内和种间病原体多样性的重要性。一种甘氏T. gamsii分离物被认为是最有希望的候选物，它能完全抑制镰刀菌的生长（100%），防止或显著减少霉菌毒素的产生（脱氧雪腐镰刀菌醇的产生最多可减少100%）。这项研究强调，麦田是一个有价值的潜在bca库，可以对抗FHB控制。

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.