Electrochemical sensing glutathione, nicotinamide adenine dinucleotide and folic acid in biological fluids using silver nanoparticles dispersed polythionine/graphene nanoflakes nanocomposite modified glassy carbon electrode

IF 6.3 3区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of the Taiwan Institute of Chemical Engineers Pub Date : 2025-08-26 DOI:10.1016/j.jtice.2025.106346

J. Kalaiyarasi , K. Pandian , S.C.B. Gopinath

{"title":"Electrochemical sensing glutathione, nicotinamide adenine dinucleotide and folic acid in biological fluids using silver nanoparticles dispersed polythionine/graphene nanoflakes nanocomposite modified glassy carbon electrode","authors":"J. Kalaiyarasi , K. Pandian , S.C.B. Gopinath","doi":"10.1016/j.jtice.2025.106346","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>A new voltammetric sensor was developed for the simultaneous detection of glutathione (GSH), nicotinamide adenine dinucleotide (NADH), and folic acid (FA) using a PTH/GNF@AgNP-modified electrode.</div></div><div><h3>Methods</h3><div>This was achieved by uniformly dispersing green-synthesized silver nanoparticles (AgNPs) onto a stable polythionine/graphene nanoflakes (PTH/GNF) nanohybrid thin film in two stages. Characterization using physicochemical and electrochemical methods confirmed the formation of the nanocomposite film. The PTH/GNF@AgNP/GCE nanocomposite exhibited an enhanced electrocatalytic oxidation of GSH, NADH, and FA within physiological pH ranges. Under optimized conditions, the sensor enabled highly selective, sensitive, and simultaneous determination of these analytes using differential pulse voltammetry (DPV). The DPV technique shows a well-defined peak potential and higher current responses compared to cyclic voltammetry. The peak current displayed a linear dependence on the concentration: 1.3 µM to 46 mM for GSH and NADH, 1.3 µM to 50 mM for FA using DPV, with detection limits of 28 nM, 24 nM, and 14 nM for GSH, NADH, and FA, respectively.</div></div><div><h3>Significant Findings</h3><div>The fabricated sensor demonstrated a wider linear range, low detection limit, improved sensitivity, good stability, and recovery, allowing for simple, selective, and precise quantitative voltammetric detection of the three analytes. The sensor successfully detected the GSH, NADH, and FA individually and simultaneously inblood serum, urine and pharmaceutical forumlations with satisfactory results.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"176 ","pages":"Article 106346"},"PeriodicalIF":6.3000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Taiwan Institute of Chemical Engineers","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876107025003979","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background

A new voltammetric sensor was developed for the simultaneous detection of glutathione (GSH), nicotinamide adenine dinucleotide (NADH), and folic acid (FA) using a PTH/GNF@AgNP-modified electrode.

Methods

This was achieved by uniformly dispersing green-synthesized silver nanoparticles (AgNPs) onto a stable polythionine/graphene nanoflakes (PTH/GNF) nanohybrid thin film in two stages. Characterization using physicochemical and electrochemical methods confirmed the formation of the nanocomposite film. The PTH/GNF@AgNP/GCE nanocomposite exhibited an enhanced electrocatalytic oxidation of GSH, NADH, and FA within physiological pH ranges. Under optimized conditions, the sensor enabled highly selective, sensitive, and simultaneous determination of these analytes using differential pulse voltammetry (DPV). The DPV technique shows a well-defined peak potential and higher current responses compared to cyclic voltammetry. The peak current displayed a linear dependence on the concentration: 1.3 µM to 46 mM for GSH and NADH, 1.3 µM to 50 mM for FA using DPV, with detection limits of 28 nM, 24 nM, and 14 nM for GSH, NADH, and FA, respectively.

Significant Findings

The fabricated sensor demonstrated a wider linear range, low detection limit, improved sensitivity, good stability, and recovery, allowing for simple, selective, and precise quantitative voltammetric detection of the three analytes. The sensor successfully detected the GSH, NADH, and FA individually and simultaneously inblood serum, urine and pharmaceutical forumlations with satisfactory results.

Abstract Image

查看原文本刊更多论文

电化学传感生物体液中的谷胱甘肽、烟酰胺腺嘌呤二核苷酸和叶酸采用纳米银粒子分散聚硫氨酸/石墨烯纳米片纳米复合修饰玻碳电极

研究了一种新型伏安传感器，采用PTH/GNF@AgNP-modified电极同时检测谷胱甘肽（GSH）、烟酰胺腺嘌呤二核苷酸（NADH）和叶酸（FA）。方法分两步将绿色合成的银纳米粒子（AgNPs）均匀分散在稳定的聚硫氨酸/石墨烯纳米片（PTH/GNF）纳米杂化薄膜上。利用物理化学和电化学方法进行表征，证实了纳米复合膜的形成。PTH/GNF@AgNP/GCE纳米复合材料在生理pH范围内表现出增强的GSH、NADH和FA的电催化氧化作用。在优化的条件下，该传感器使用差分脉冲伏安法（DPV）实现了高选择性、灵敏度和同时测定这些分析物。与循环伏安法相比，DPV技术具有明确的峰电位和更高的电流响应。峰值电流与浓度呈线性关系：DPV法测定GSH和NADH为1.3µM ~ 46 mM， FA为1.3µM ~ 50 mM， GSH、NADH和FA的检测限分别为28 nM、24 nM和14 nM。该传感器具有较宽的线性范围、较低的检测限、提高的灵敏度、良好的稳定性和回收率，可以对三种分析物进行简单、选择性和精确的定量伏安检测。该传感器成功地单独和同时检测血清、尿液和药物制剂中的GSH、NADH和FA，结果令人满意。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Taiwan Institute of Chemical Engineers 工程技术-工程：化工

CiteScore

9.10

自引率

14.00%

发文量

362

审稿时长

35 days

期刊介绍： Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.