Effect of autochthonous live yeasts on survival, growth, digestive functionality, and innate immune response in cobia (Rachycentron canadum) larvae

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-08-26 DOI:10.1016/j.aqrep.2025.103051

Samira Reinoso , María Soledad Gutiérrez , Cristóbal Domínguez-Borbor , Melania Vargas , Irma Betancourt , Wilfrido Argüello-Guevara , Stanislaus Sonnenholzner , Carlos Alfonso Álvarez-González , Enric Gisbert , Paola Navarrete

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引用次数: 0

Abstract

The use of host-derived probiotic yeasts is a promising strategy to enhance larval performance, but their effects during and after supplementation remain poorly understood. This study evaluated the effects of three autochthonous yeast strains: Candida haemuloni C27, Debaryomyces hansenii C10, and D. hansenii C28, isolated from cobia (Rachycentron canadum). A well-known strain, D. hansenii CBS8339 (isolated from rainbow trout), was included for comparison. Larvae were fed for 14 days (2–15 days post-hatch, dph) with rotifers and Artemia metanauplii enriched with each yeast strain. A total of 75,000 larvae (2 dph; ∼3 mm) were randomly distributed into 15 tanks, with three replicates assigned to each of five groups: three groups received yeasts, one group was supplemented with D. hansenii CBS8339, and one control group received non-enriched live food. Digestive enzyme activity, larval growth, and yeast persistence in the gastrointestinal tract were assessed during (6 and 12 dph) and after (18 and 24 dph) supplementation. Survival and innate immune gene expression were evaluated at 24 dph. The results showed that all strains except D. hansenii C10 remained viable in the larval gut until 24 dph. Larvae fed C. haemuloni C27 and D. hansenii CBS8339 showed higher acid protease activity during supplementation. After supplementation, D. hansenii C28 enhanced the activity of eight out of nine digestive enzymes analyzed, while all yeast-fed groups showed increased α-amylase and lipase activity. C. haemuloni C27 upregulated il-1β and tnf-α expression. Survival was improved in larvae supplemented with C. haemuloni C27 and D. hansenii C28 (∼31 %) compared to the control (∼22 %). No differences in growth or intestinal histology were detected between treatments. These findings demonstrate that C. haemuloni C27 and D. hansenii C28 exert beneficial effects in cobia larvae by enhancing survival, digestive function, and immune response with lasting effects likely due to their persistence in the gut.

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本地活酵母对军曹幼虫生存、生长、消化功能和先天免疫反应的影响

使用宿主衍生的益生菌酵母是提高幼虫生产性能的一种很有前途的策略，但它们在补充期间和之后的效果仍然知之甚少。本研究评估了从土豆杉（Rachycentron canadum）中分离的三种本地酵母菌菌株：haemuloni假丝酵母C27、hansenii Debaryomyces C10和D. hansenii C28的效果。一种众所周知的菌株，D. hansenii CBS8339（从虹鳟中分离）被纳入比较。孵育14 d（孵化后2 ~ 15 d， dph），分别饲喂各酵母菌株富集的轮虫和后黄蒿。将75000只幼虫（2 dph; ~ 3 mm）随机分配到15个鱼缸中，每组设3个重复，分为5个组：3组添加酵母，1组添加粪肠杆菌CBS8339, 1组添加未添加的活饲料。在（6和12 dph）和（18和24 dph）补充后，评估消化道消化酶活性、幼虫生长和酵母菌持久性。在24 dph时评估生存和先天免疫基因表达。结果表明，除粪肠杆菌C10外，其余菌株均可在幼虫肠道内存活至24 dph。饲喂haemuloni C27和D. hansenii CBS8339的幼虫显示出较高的酸性蛋白酶活性。添加酵母C28后，9种消化酶中8种酶活性均有所提高，而所有酵母饲喂组α-淀粉酶和脂肪酶活性均有所提高。haemuloni C27上调il-1β和tnf-α的表达。与对照组（~ 22 %）相比，添加haemuloni C27和D. hansenii C28的幼虫存活率提高了（~ 31 %）。两种治疗之间在生长和肠道组织学上没有发现差异。这些研究结果表明，C. haemuloni C27和D. hansenii C28通过提高cobia幼虫的存活率、消化功能和免疫反应而对cobia幼虫产生有益的影响，并且由于它们在肠道中的持久性，可能会产生持久的影响。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.